Crystallization (Comment) | Organism |
---|---|
molecular dynamics simulations suggest a nucleophilic attack of the deprotonated sulfur of Cys112 at the carbonyl carbon of ACoA and a switch in the protonation pattern of His257 whereby Ndelta is protonated and the proton of Nepsilon is shifted to the sulfur of CoA during the reaction. In the N287A mutant anthraniloyl remains covalently bound to C112, further supporting that N87 does not take part in the cleavage of anthraniloyl-CoA | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C112A | inactive | Pseudomonas aeruginosa |
C112S | about 20% of wild-type activity | Pseudomonas aeruginosa |
H257F | inactive | Pseudomonas aeruginosa |
N287A | inactive, anthraniloyl remains covalently bound to C112, further supporting that N87 does not take part in the cleavage of anthraniloyl-CoA | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P20582 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PsqD | - |
Pseudomonas aeruginosa |