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Literature summary for 2.3.1.230 extracted from

  • Pistorius, D.; Ullrich, A.; Lucas, S.; Hartmann, R.W.; Kazmaier, U.; Müller, R.
    Biosynthesis of 2-Alkyl-4(1H)-quinolones in Pseudomonas aeruginosa: potential for therapeutic interference with pathogenicity (2011), Chembiochem, 12, 850-853.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-(4-bromo-3-diethylsulfamoylbenzoylamino)benzoate
-
Pseudomonas aeruginosa
2-[(2-phenoxybiphenyl-4-carbonyl)amino]benzoate
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0487
-
malonyl-CoA pH and temperature not specified in the publication Pseudomonas aeruginosa
0.599
-
2-aminobenzoyl-CoA pH and temperature not specified in the publication Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38440
-
HPLC mass spectrometry Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + 2-aminobenzoyl-CoA Pseudomonas aeruginosa the enzyme is involved in the biosynthesis of the signal molecules 2-heptyl-3-hydroxy-4(1H)-quinolone and 4-hydroxy-2(1H)-quinolone. These compounds are part of the quorum-sensing systems that is based on the production and release of small signaling molecules, called autoinducers, that increase in concentration as a function of cell density and activate corresponding transcriptional regulators after a threshold concentration has been reached 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxodecanoate + 2-aminobenzoyl-CoA
-
Pseudomonas aeruginosa CoA + 2-heptyl-4(1H)-quinolone + CO2 + H2O
-
?
3-oxodecanoyl-CoA + 2-aminobenzoyl-CoA high concentrations and a longer incubation time is required in comparison to the free 3-oxodecanoic acid Pseudomonas aeruginosa 2 CoA + 2-heptyl-3-hydroxy-4(1H)-quinolone + CO2
-
?
3-oxodecanoyl-N-acetylcysteamine + 2-aminobenzoyl-CoA high concentrations and a longer incubation time is required in comparison to the free 3-oxodecanoic acid Pseudomonas aeruginosa N-acetylcysteamine + 2-heptyl-3-hydroxy-4(1H)-quinolone + CO2
-
?
3-oxohexanoate + 2-aminobenzoyl-CoA
-
Pseudomonas aeruginosa CoA + 2-propyl-4(1H)-quinolone + CO2 + H2O
-
?
3-oxopentanoate + 2-aminobenzoyl-CoA
-
Pseudomonas aeruginosa CoA + 2-ethyl-4(1H)-quinolone + CO2 + H2O
-
?
malonyl-CoA + 2-aminobenzoyl-CoA the enzyme is involved in the biosynthesis of the signal molecules 2-heptyl-3-hydroxy-4(1H)-quinolone and 4-hydroxy-2(1H)-quinolone. These compounds are part of the quorum-sensing systems that is based on the production and release of small signaling molecules, called autoinducers, that increase in concentration as a function of cell density and activate corresponding transcriptional regulators after a threshold concentration has been reached Pseudomonas aeruginosa 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2
-
?
malonyl-CoA + 2-aminobenzoyl-CoA catalyzes the decarboxylative Claisen condensation with malonyl-CoA. Pseudomonas aeruginosa 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.035
-
pH and temperature not specified in the publication Pseudomonas aeruginosa 2-[(2-phenoxybiphenyl-4-carbonyl)amino]benzoate
0.065
-
pH and temperature not specified in the publication Pseudomonas aeruginosa 2-(4-bromo-3-diethylsulfamoylbenzoylamino)benzoate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
2-aminobenzoyl-CoA pH and temperature not specified in the publication Pseudomonas aeruginosa
69
-
malonyl-CoA pH and temperature not specified in the publication Pseudomonas aeruginosa