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Literature summary for 2.3.1.230 extracted from

  • Steinbach, A.; Maurer, C.K.; Weidel, E.; Henn, C.; Brengel, C.; Hartmann, R.W.; Negri, M.
    Molecular basis of HHQ biosynthesis: molecular dynamics simulations, enzyme kinetic and surface plasmon resonance studies (2013), BMC Biophys., 6, 10.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.230

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
S317F inactive mutant enzyme Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.875
-
 2-aminobenzoyl-CoA pH 7, 37°C Pseudomonas aeruginosa
1.3
-
 3-oxodecanoate pH 7, 37°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + 2-aminobenzoyl-CoA Pseudomonas aeruginosa 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. The enzyme is involved in the biosynthesis of the Pseudomonas Quinolone Signal 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 i.e. 2,4-dihydroxyquinoline ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxodecanoate + 2-aminobenzoyl-CoA
-
 Pseudomonas aeruginosa CoA + 2-heptyl-4-hydroxyquinoline + CO2 + H2O
-
 ?
malonyl-CoA + 2-aminobenzoyl-CoA 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. The enzyme is involved in the biosynthesis of the Pseudomonas Quinolone Signal Pseudomonas aeruginosa 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 i.e. 2,4-dihydroxyquinoline ?
malonyl-CoA + 2-aminobenzoyl-CoA 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA. Ping-pong mechanism for PqsD with 2-aminobenzoyl-CoA as first substrate. Trajectory analysis of different PqsD complexes evidences ligand-dependent induced-fit motions affecting the modified 2-aminobenzoyl-CoA funnel access to the exposure of a secondary channel. A tunnel-network is formed in which Ser317 plays an important role by binding to both substrates Pseudomonas aeruginosa 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 i.e. 2,4-dihydroxyquinoline ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0165
-
 2-aminobenzoyl-CoA pH 7, 37°C Pseudomonas aeruginosa
0.0165
-
 3-oxodecanoate pH 7, 37°C Pseudomonas aeruginosa