Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a potential therapeutic targets for the regulation of immune and inflammatory disorders | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene LPCAT3, quantitative real-time RT-PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | LPCAT3 isozyme knockdown to about 25% activity in U-937 cells by siRNA. LPCAT3 siRNA-transfected cells show a spindle-shaped. morphology similar to that of M1-polarized macrophages, whereas control siRNAtransfected cells show a rounded morphology typical ofM2-polarized macrophages. Knockdown of LPCAT3 in phorbol ester-treated U-937 cells decreases LPCAT activity toward linoleoyl-CoA and arachidonoyl-CoA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lipopolysaccharide | suppresses the enzyme activity of isozyme LPCAT3 toward linoleoyl-CoA and arachidonoyl-CoA in phorbol 12-myristate 13-acetate-treated U-937 cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | Homo sapiens | - |
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P1A2 | LPCAT3, one of four isozymes, gene LPCAT3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukemia cell | - |
Homo sapiens | - |
macrophage | - |
Homo sapiens | - |
monocyte | - |
Homo sapiens | - |
U-937 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Homo sapiens | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
? | |
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Homo sapiens | CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine | - |
? | |
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Homo sapiens | CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LPCAT3 | - |
Homo sapiens |
lysophosphatidylcholine acyltransferase 3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | lipopolysaccharide significantly downregulates the mRNA expression of isozyme LPCAT3 | down |
General Information | Comment | Organism |
---|---|---|
malfunction | LPCAT3 knockdown induces a spindle-shaped morphology typical of M1-polarized macrophages, increases the secretion of CXCL10, and decreases the levels of CD206 in interleukin-4-activated U-937 cells. Knockdown of LPCAT3 shifts the differentiation of phorbol ester-treated U-937 cells to M1-polarized macrophages. Decrease in the content of phosphatidylcholine containing linoleic acid or arachidonic acid in biological membranes caused by the suppression of LPCAT3 may influencemembrane fluidity, curvature and function | Homo sapiens |
physiological function | lysophospholipid acyltransferases (LPLATs) regulate the diversification of fatty acid composition in biological membranes. Lysophosphatidylcholine acyltransferases (LPCATs) are members of the LPLATs that play a role in inflammatory responses. LPCAT3 is reported to be a major contributor to increase polyunsaturated fatty acids, including linoleic acid and arachidonic acid, and is associated with inflammatory responses in human primary macrophages, and the enzyme LPCAT3 plays an important role in M1/M2-macrophage polarization, analysis of the underlying mechanism | Homo sapiens |