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Literature summary for 2.3.1.227 extracted from

  • Albermann, C.; Beuttler, H.
    Identification of the GDP-N-acetyl-d-perosamine producing enzymes from Escherichia coli O157:H7 (2008), FEBS Lett., 582, 479-484.
    View publication on PubMed
Search for more literature for 2.3.1.227

Cloned(Commentary)

Cloned (Comment) Organism
expression as a His-tagged fusion protein in Escherichia coli BL21 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 GDP-4-amino-4,6-dideoxy-alpha-D-mannose pH 8.0, 37°C Escherichia coli
1.8
-
 acetyl-CoA pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26260
-
 3 * 26260, His-tag PerB, SDS-PAGE Escherichia coli
68000
-
 His-tag PerB, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose Escherichia coli GDP-4-amino-4,6-dideoxy-alpha-D-mannose (i.e. GDP-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged fusion protein Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 30 days, His-tag PerB, 5% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose GDP-4-amino-4,6-dideoxy-alpha-D-mannose (i.e. GDP-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine Escherichia coli CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine ?
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose GDP-4-amino-4,6-dideoxy-alpha-D-mannose i.e. GDP-alpha-D-perosamine Escherichia coli CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine ?

Subunits

Subunits Comment Organism
trimer 3 * 26260, His-tag PerB, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
acetyl-CoA:GDP-D-perosamine N-acetyltransferase
-
 Escherichia coli
PerB
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 His-tag PerB, 20 min, 24% loss of activity Escherichia coli
75
-
 His-tag PerB, 20 min, 90% loss of activity Escherichia coli
80
-
 His-tag PerB, 10 min, complete inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 His-tag PerB protein Escherichia coli

General Information

General Information Comment Organism
physiological function GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose (i.e. GDP-N-acetyl-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine Escherichia coli