Application | Comment | Organism |
---|---|---|
medicine | carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics | Pectobacterium carotovorum |
synthesis | carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics | Pectobacterium carotovorum |
Protein Variants | Comment | Organism |
---|---|---|
H229A | the CarB variant is able to form (2S,7S)-7-(carboxymethyl)-azepane-2-carboxylic acid from malonyl-CoA and L-aminopimelate semialdehyde in a yield of about 3 times higher than that obtained with wild-type CarB | Pectobacterium carotovorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | - |
Pectobacterium carotovorum | CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? | |
malonyl-CoA + L-2-aminopimelate semialdehyde | the CarB His229Ala variant is able to form the target compound in a yield of about 3 times higher than that obtained with wild-type CarB | Pectobacterium carotovorum | CoA + (2S,7S)-7-(carboxymethyl)-azepane-2-carboxylic acid + CO2 | - |
? | |
additional information | no activity with L-2-aminosuberate semialdehyde (wild-type enzyme and mutant enzyme H229A) | Pectobacterium carotovorum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CarB | - |
Pectobacterium carotovorum |