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Literature summary for 2.3.1.225 extracted from

  • Gonzalez Montoro, A.; Chumpen Ramirez, S.; Valdez Taubas, J.
    The canonical DHHC motif is not absolutely required for the activity of the yeast S-acyltransferases Swf1 and Pfa4 (2015), J. Biol. Chem., 290, 22448-22459.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.225

Protein Variants

Protein Variants Comment Organism
C164R substitution of the conserved cysteine in the DHHC motif. Overexpression of this mutant allows to partially complement an isoform Swf1 gene deletion and to acylate the Swf1 substrates Tlg1, Syn8, and Snc1. Mutant protein is not acylated Saccharomyces cerevisiae
H162Q substitution in the DHHC motif, mutant is partially active Saccharomyces cerevisiae
H162Q/C164R substitutions in the DHHC motif, mutant is inactive Saccharomyces cerevisiae
additional information substitution of the conserved cysteine in the DHHC motif to DHHA or DHHR still allows for acylation of substrate Chs3. Mutant protein is not acylated Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q04629
-
-
Saccharomyces cerevisiae Q12006
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
palmitoylation enzyme undergoes autoacylation Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + [Chs3]-L-cysteine
-
 Saccharomyces cerevisiae [Chs3]-S-palmitoyl-L-cysteine + CoA
-
 ?
palmitoyl-CoA + [Pfa4]-L-cysteine autoacylation Saccharomyces cerevisiae [Pfa4]-S-palmitoyl-L-cysteine + CoA
-
 ?
palmitoyl-CoA + [Snc1]-L-cysteine
-
 Saccharomyces cerevisiae [Snc1]-S-palmitoyl-L-cysteine + CoA
-
 ?
palmitoyl-CoA + [Swf1]-L-cysteine autoacylation Saccharomyces cerevisiae [Swf1]-S-palmitoyl-L-cysteine + CoA
-
 ?
palmitoyl-CoA + [Syn8]-L-cysteine
-
 Saccharomyces cerevisiae [Syn8]-S-palmitoyl-L-cysteine + CoA
-
 ?
palmitoyl-CoA + [Tlg1]-L-cysteine
-
 Saccharomyces cerevisiae [Tlg1]-S-palmitoyl-L-cysteine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
Pfa4
-
 Saccharomyces cerevisiae
Swf1
-
 Saccharomyces cerevisiae