Cloned (Comment) | Organism |
---|---|
DHHC16, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC16 in HEK293Acells | Homo sapiens |
DHHC2, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC2 in HEK293Acells | Homo sapiens |
DHHC4, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC4 in HEK293A cells, quantitative expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | DHHC2 targeting with siRNA leads to Lck partial delocalization from the membranes and moving to the cytosol | Homo sapiens |
additional information | DHHC4 targeting with siRNA leads to Lck partial delocalization from the membranes and moving to the cytosol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | DHHC2 is present in at least some areas of the endoplasmic reticulum and Golgi | Homo sapiens | 5783 | - |
Golgi membrane | DHHC2 is present in at least some areas of the endoplasmic reticulum and Golgi | Homo sapiens | 139 | - |
membrane | associated | Homo sapiens | 16020 | - |
additional information | the DHHC protein is not associated with early endosomes | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [protein]-L-cysteine | Homo sapiens | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q969W1 | ZDHC16, gene ZDHHC116; gene ZDHHC2 | - |
Homo sapiens | Q9NPG8 | ZDHC4, gene ZDHHC4; gene ZDHHC4 | - |
Homo sapiens | Q9UIJ5 | ZDHC2, gene ZDHHC2; gene ZDHHC2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
JURKAT cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [protein]-L-cysteine | - |
Homo sapiens | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
DHHC16 | - |
Homo sapiens |
DHHC2 | - |
Homo sapiens |
DHHC4 | - |
Homo sapiens |
Pat | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | overexpression of DHHC2 causes increased S-acylation of LckN10-GFP. In resting Jurkat T cells, endogenous DHHC2 and Lck, a non-receptor tyrosine kinase of the Src family, are in close proximity to each other at the cell periphery, but completely non-overlapping, DHHC2 is a PAT for Lck in vivo | Homo sapiens |
additional information | overexpression of DHHC4 does not cause increased S-acylation of LckN10-GFP | Homo sapiens |