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Literature summary for 2.3.1.224 extracted from

  • Nam, K.H.; Dudareva, N.; Pichersky, E.
    Characterization of benzylalcohol acetyltransferases in scented and non-scented Clarkia species (1999), Plant Cell Physiol., 40, 916-923.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Clarkia breweri
expressed in Escherichia coli BL21 cells Clarkia concinna

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
acetyl-CoA in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C Clarkia breweri
0.12
-
benzyl alcohol isoform BEAT-1, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C Clarkia concinna
0.12
-
benzyl alcohol isoform BEAT-2, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C Clarkia breweri
0.24
-
benzyl alcohol isoform BEAT-1, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C Clarkia breweri
0.34
-
benzyl alcohol isoform BEAT-2, in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30°C Clarkia concinna

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + benzyl alcohol Clarkia breweri 100% activity CoA + benzyl acetate
-
?
acetyl-CoA + benzyl alcohol Clarkia concinna 100% activity CoA + benzyl acetate
-
?

Organism

Organism UniProt Comment Textmining
Clarkia breweri
-
-
-
Clarkia concinna
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Clarkia breweri
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Clarkia concinna

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Clarkia breweri
-
flower
-
Clarkia concinna
-
petal
-
Clarkia breweri
-
petal
-
Clarkia concinna
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-(naphthalen-1-yl)ethanol 139% activity compared to benzyl alcohol with isoform BEAT-1, 93% activity compared to benzyl alcohol with isoform BEAT-2, 217% activity compared to benzyl alcohol with isoform BEAT-3 Clarkia concinna CoA + 2-(naphthalen-1-yl)ethyl acetate
-
?
acetyl-CoA + 2-phenylethanol 11% activity compared to benzyl alcohol with isoform BEAT-1, 21% activity compared to benzyl alcohol with isoform BEAT-2, 131% activity compared to benzyl alcohol with isoform BEAT-3 Clarkia concinna CoA + 2-phenylethyl acetate
-
?
acetyl-CoA + 2-phenylethanol 28% activity compared to benzyl alcohol with isoform BEAT-1, 40% activity compared to benzyl alcohol with isoform BEAT-2 Clarkia breweri CoA + 2-phenylethyl acetate
-
?
acetyl-CoA + 3-cis-hexene-1-ol 102% activity compared to benzyl alcohol with isoform BEAT-1, 102% activity compared to benzyl alcohol with isoform BEAT-2 Clarkia breweri CoA + (3Z)-hex-3-en-1-yl acetate
-
?
acetyl-CoA + 3-cis-hexene-1-ol 70% activity compared to benzyl alcohol with isoform BEAT-1, 128% activity compared to benzyl alcohol with isoform BEAT-2, 66% activity compared to benzyl alcohol with isoform BEAT-3 Clarkia concinna CoA + (3Z)-hex-3-en-1-yl acetate
-
?
acetyl-CoA + benzyl alcohol 100% activity Clarkia breweri CoA + benzyl acetate
-
?
acetyl-CoA + benzyl alcohol 100% activity Clarkia concinna CoA + benzyl acetate
-
?
acetyl-CoA + cinnamyl alcohol 147% activity compared to benzyl alcohol with isoform BEAT-1, 162% activity compared to benzyl alcohol with isoform BEAT-2, 838% activity compared to benzyl alcohol with isoform BEAT-3 Clarkia concinna CoA + cinnamyl acetate
-
?
acetyl-CoA + cinnamyl alcohol 97% activity compared to benzyl alcohol with isoform BEAT-1, 122% activity compared to benzyl alcohol with isoform BEAT-2 Clarkia breweri CoA + cinnamyl acetate
-
?
acetyl-CoA + heptanol 143% activity compared to benzyl alcohol with isoform BEAT-1, 150% activity compared to benzyl alcohol with isoform BEAT-2 Clarkia breweri CoA + heptyl acetate
-
?
acetyl-CoA + heptanol 498% activity compared to benzyl alcohol with isoform BEAT-1, 528% activity compared to benzyl alcohol with isoform BEAT-2, 502% activity compared to benzyl alcohol with isoform BEAT-3 Clarkia concinna CoA + heptyl acetate
-
?
acetyl-CoA + naphthalene ethanol 57% activity compared to benzyl alcohol with isoform BEAT-1, 50% activity compared to benzyl alcohol with isoform BEAT-2 Clarkia breweri ?
-
?

Synonyms

Synonyms Comment Organism
acetyl-CoA:benzylalcohol acetyltransferase
-
Clarkia breweri
acetyl-CoA:benzylalcohol acetyltransferase
-
Clarkia concinna
BEAT-1 isoform Clarkia breweri
BEAT-1 isoform Clarkia concinna
BEAT-2 isoform Clarkia breweri
BEAT-2 isoform Clarkia concinna
BEAT-3 isoform Clarkia concinna
benzylalcohol acetyltransferase
-
Clarkia breweri
benzylalcohol acetyltransferase
-
Clarkia concinna