Cloned (Comment) | Organism |
---|---|
gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli | Aspergillus parasiticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] | Aspergillus parasiticus | - |
7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus parasiticus | - |
- |
- |
Aspergillus parasiticus | - |
gene pksA | - |
Purification (Comment) | Organism |
---|---|
recombinant C- or N-terminally His6-tagged mono-, di-, and tridomains protein fragments from Escherichia coli by metal affinity cromatography | Aspergillus parasiticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O | a product template domain unites with the ketosynthase and thioesterase in this IPKS system to assemble precisely seven malonyl-derived building blocks to a hexanoyl starter unit and mediate a specific cyclization cascade. These mechanistic features are general for IPKS-catalyzed production of aromatic polyketides | Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] | - |
Aspergillus parasiticus | 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | domain structure, and dissection of domains, interactions, overview | Aspergillus parasiticus |
Synonyms | Comment | Organism |
---|---|---|
norsolorinic acid anthrone synthase | - |
Aspergillus parasiticus |
PksA | - |
Aspergillus parasiticus |
polyketide synthase A | - |
Aspergillus parasiticus |
General Information | Comment | Organism |
---|---|---|
metabolism | PksA is involved in the synthesis of norsolorinic acid, an intermediate in the biosynthesis of the environmental carcinogen aflatoxin B1 | Aspergillus parasiticus |
physiological function | PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases, IPKSs | Aspergillus parasiticus |