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Literature summary for 2.3.1.22 extracted from

  • Jin, Y.; McFie, P.J.; Banman, S.L.; Brandt, C.; Stone, S.J.
    Diacylglycerol acyltransferase-2 (DGAT2) and monoacylglycerol acyltransferase-2 (MGAT2) interact to promote triacylglycerol synthesis (2014), J. Biol. Chem., 289, 28237-28248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene MOGAT, co-expression of enzyme MGAT2 and acyl CoA:1,2-diacylglycerol acyltransferase, DGAT2, in HEK-293T and in COS-7 cells and co-localization in the endoplasmic reticulum and on lipid droplets Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
Homo sapiens 5789
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, about, SDS-PAGE Homo sapiens
80000
-
the enzyme exists as both a homodimer and homotetramer, cross-linking Homo sapiens
160000
-
the enzyme exists as both a homodimer and homotetramer, cross-linking Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 2-acylglycerol Homo sapiens
-
CoA + diacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q3SYC2 gene MOGAT
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 2-acylglycerol
-
Homo sapiens CoA + diacylglycerol
-
?
N-(7-nitro-2-1,3-benzoxadiazol-4-yl)amino-palmitoyl-CoA + 2-oleoylglycerol fluorescent-labeled substrate Homo sapiens CoA + N-(7-nitro-2-1,3-benzoxadiazol-4-yl)amino-palmitoyl-diacylglycerol
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, about, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Mgat2
-
Homo sapiens
monoacylglycerol acyltransferase-2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the evolutionarily conserved acyltransferase gene family. All DGAT2 family members, including monoacylglycerol acyltransferases (MGAT)1-3 and wax synthases 1 and 2, contain a highly conserved four amino acid sequence - histidine-proline-histidine-glycine Homo sapiens
metabolism the enzyme interacts with the acyl CoA:1,2-diacylglycerol acyltransferase (DGAT)-2, an endoplasmic reticulum integral membrane protein that catalyzes triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. Deletion mutagenesis shows that the interaction with MGAT2 is dependent on the two transmembrane domains of DGAT2. The interaction of the enzyme and DGAT2 serves to channel lipid substrates efficiently for triacylglycerol biosynthesis Homo sapiens
physiological function in the intestine, the majority of diacylglycerol is produced by the MGAT pathway via monoacylglycerol acyltransferase-2, MGAT2, which catalyzes the synthesis of diacylglycerol in an acyl CoA-dependent manner using 2-monoacylglycerol as an acyl acceptor. Co-expression of acyl CoA:1,2-diacylglycerol acyltransferase, DGAT2, and monoacylglycerol acyltransferase MGAT2 stimulates triacylglycerol storage Homo sapiens