Cloned (Comment) | Organism |
---|---|
gene MOGAT, co-expression of enzyme MGAT2 and acyl CoA:1,2-diacylglycerol acyltransferase, DGAT2, in HEK-293T and in COS-7 cells and co-localization in the endoplasmic reticulum and on lipid droplets | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, about, SDS-PAGE | Homo sapiens |
80000 | - |
the enzyme exists as both a homodimer and homotetramer, cross-linking | Homo sapiens |
160000 | - |
the enzyme exists as both a homodimer and homotetramer, cross-linking | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 2-acylglycerol | Homo sapiens | - |
CoA + diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q3SYC2 | gene MOGAT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 2-acylglycerol | - |
Homo sapiens | CoA + diacylglycerol | - |
? | |
N-(7-nitro-2-1,3-benzoxadiazol-4-yl)amino-palmitoyl-CoA + 2-oleoylglycerol | fluorescent-labeled substrate | Homo sapiens | CoA + N-(7-nitro-2-1,3-benzoxadiazol-4-yl)amino-palmitoyl-diacylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, about, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Mgat2 | - |
Homo sapiens |
monoacylglycerol acyltransferase-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the evolutionarily conserved acyltransferase gene family. All DGAT2 family members, including monoacylglycerol acyltransferases (MGAT)1-3 and wax synthases 1 and 2, contain a highly conserved four amino acid sequence - histidine-proline-histidine-glycine | Homo sapiens |
metabolism | the enzyme interacts with the acyl CoA:1,2-diacylglycerol acyltransferase (DGAT)-2, an endoplasmic reticulum integral membrane protein that catalyzes triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. Deletion mutagenesis shows that the interaction with MGAT2 is dependent on the two transmembrane domains of DGAT2. The interaction of the enzyme and DGAT2 serves to channel lipid substrates efficiently for triacylglycerol biosynthesis | Homo sapiens |
physiological function | in the intestine, the majority of diacylglycerol is produced by the MGAT pathway via monoacylglycerol acyltransferase-2, MGAT2, which catalyzes the synthesis of diacylglycerol in an acyl CoA-dependent manner using 2-monoacylglycerol as an acyl acceptor. Co-expression of acyl CoA:1,2-diacylglycerol acyltransferase, DGAT2, and monoacylglycerol acyltransferase MGAT2 stimulates triacylglycerol storage | Homo sapiens |