Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.22 extracted from

  • Coleman, R.A.; Haynes, E.B.
    Monoacylglycerol acyltransferase. Evidence that the activities from rat intestine and suckling liver are tissue-specific isoenzymes (1986), J. Biol. Chem., 261, 224-228.
    View publication on PubMed
Search for more literature for 2.3.1.22

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation, liver, below 1 mg/ml, inhibitory above, not intestine Rattus norvegicus
deoxycholate stimulates enzyme from intestinal mucosa Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzene sulfonic acid 3 mM, 80% inactivation of liver enzyme, 45% inactivation of intestinal enzyme, palmitate partly protects, enzyme from intestine is less sensitive than enzyme from liver Rattus norvegicus
2-monooleoylglycerol high concentration Rattus norvegicus
acetone 10%, liver Rattus norvegicus
bovine serum albumin activation below 1 mg/ml Rattus norvegicus
deoxycholate inhibition of liver enzyme Rattus norvegicus
diethyldicarbonate 0.04 mM, 50% inhibition, 0.05 mM palmitate partly protects, completely reversible by 50 mM hydroxylamine, enzyme from intestine is less sensitive than enzyme from liver Rattus norvegicus
EDTA weak Rattus norvegicus
HgCl2 weak Rattus norvegicus
MnCl2 2.5 mM, 86% inhibition of the intestinal enzyme, 40% inhibition of the enzyme from suckling liver Rattus norvegicus
palmitoyl-CoA high concentration Rattus norvegicus
PCMB
-
 Rattus norvegicus
taurocholate
-
 Rattus norvegicus
Triton X-100
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + sn-2-monoacylglycerol Rattus norvegicus involved in synthesis of triacylglycerols CoA + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Zivic Miller SD-strain
-

Source Tissue

Source Tissue Comment Organism Textmining
liver neonatal Rattus norvegicus
-
additional information tissue specific isozymes Rattus norvegicus
-
small intestine mucosa
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + sn-2-monoacylglycerol involved in synthesis of triacylglycerols Rattus norvegicus CoA + diacylglycerol
-
 ?
fatty acyl-CoA + sn-2-monoacylglycerol
-
 Rattus norvegicus CoA + 1,2-diacylglycerol
-
 ?
palmitoyl-CoA + rac-1-monooleoylglycerol intestine, at 78% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + ?
-
 ?
palmitoyl-CoA + rac-1-monooleoylglycerol ether intestine, at 45% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + ?
-
 ?
palmitoyl-CoA + sn-2-monooleoylglycerol intestine, at 52% the rate of sn-2-monooleoylglycerol acylation Rattus norvegicus CoA + 1(3)-palmitoyl-2-oleoylglycerol
-
 ?
palmitoyl-CoA + sn-2-monooleoylglycerol liver enzyme highly specific for sn-2-monooleoylglycerol Rattus norvegicus CoA + 1(3)-palmitoyl-2-oleoylglycerol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 12 min, microsomes from liver retain 90% of the initial activity Rattus norvegicus
51
-
 12 min, microsomes from liver lose 23% of the initial activity, microsomes from intestinal mucosa lose 88.5% of control activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Rattus norvegicus