Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Curcuma longa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | DCS is an allosteric enzyme. Homotropic activation decreases the activity as the substrate concentration decreases more quickly than those exhibiting Michaelis-Menten-type kinetics. Kinetic analysis of DCS, overview | Curcuma longa | |
0.0084 | - |
malonyl-CoA | pH 7.5, 37°C, recombinant enzyme | Curcuma longa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA | Curcuma longa | - |
(4-coumaroyl)acetyl-CoA + CO2 + CoA | - |
? | |
feruloyl-CoA + malonyl-CoA | Curcuma longa | - |
feruloylacetyl-CoA + CO2 + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma longa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma longa | - |
rhizome | - |
Curcuma longa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA | - |
Curcuma longa | (4-coumaroyl)acetyl-CoA + CO2 + CoA | - |
? | |
feruloyl-CoA + malonyl-CoA | - |
Curcuma longa | feruloylacetyl-CoA + CO2 + CoA | - |
? | |
feruloyl-CoA + malonyl-CoA | - |
Curcuma longa | feruloylacetyl-CoA + CO2 + CoA | product identification by LC-ESI MS/MS | ? |
Synonyms | Comment | Organism |
---|---|---|
DCS | - |
Curcuma longa |
diketide-CoA synthase | - |
Curcuma longa |
phenylpropanoyl-diketide-CoA synthase | - |
Curcuma longa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Curcuma longa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0112 | - |
malonyl-CoA | pH 7.5, 37°C, recombinant enzyme | Curcuma longa | |
0.02 | - |
feruloyl-CoA | pH 7.5, 37°C, recombinant enzyme | Curcuma longa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Curcuma longa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Type III polyketide synthase family, but DCS is a unique type III PKS that releases the product as a CoA-bound form | Curcuma longa |
metabolism | the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview | Curcuma longa |
additional information | DCS can collaborate with curcumin synthase, CURS, EC 2.3.1.217, which uses the reaction product feruloylacetyl-CoA for formation of curcumin, overview | Curcuma longa |