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Literature summary for 2.3.1.217 extracted from

  • Katsuyama, Y.; Kita, T.; Funa, N.; Horinouchi, S.
    Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa (2009), J. Biol. Chem., 284, 11160-11170.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.217

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis of CURS, overview Curcuma longa
0.018
-
 feruloyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl-CoA + feruloylacetyl-CoA + H2O Curcuma longa
-
 2 CoA + curcumin + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Curcuma longa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Curcuma longa
-
rhizome
-
 Curcuma longa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O low activity Curcuma longa CoA + dicinnamoylmethane + N-acetylcysteamine
-
 ?
feruloyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
 Curcuma longa CoA + cinnamoylferuloylmethane + N-acetylcysteamine + CO2
-
 ?
feruloyl-CoA + feruloylacetyl-CoA + H2O
-
 Curcuma longa 2 CoA + curcumin + CO2
-
 ?
feruloyl-CoA + feruloylacetyl-CoA + H2O feruloyl-CoA is the preferred starter substrate Curcuma longa 2 CoA + curcumin + CO2 product identification by LC-ESI MS/MS ?
additional information the enzyme can also convert diketide-CoA esters into 2-oxoacids and CoA, which are then converted into curcuminoids. For activity assays, cinnamoyl-CoA, 4-coumaroyl-CoA, or feruloyl-CoA are used as starter substrates and malonyl-CoA or cinnamoyl-diketide-N-acetylcysteamine as extender substrates, cf. EC 2.3.1.218 and EC 2.31.219 Curcuma longa ?
-
 ?

Synonyms

Synonyms Comment Organism
curcumin synthase
-
 Curcuma longa
CURS
-
 Curcuma longa
CURS1
-
 Curcuma longa
CURS2
-
 Curcuma longa
CURS3
-
 Curcuma longa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Curcuma longa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0183
-
 feruloyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Curcuma longa

General Information

General Information Comment Organism
evolution the enzyme belongs to the Type III polyketide synthase family Curcuma longa
metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
additional information CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview Curcuma longa