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Literature summary for 2.3.1.212 extracted from

  • Borejsza-Wysocki, W.; Hrazdina, G.
    Aromatic polyketide synthases (purification, characterization, and antibody development to benzalacetone synthase from raspberry fruits) (1996), Plant Physiol., 110, 791-799.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.212

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 malonyl-CoA pH 8.0, 30°C Rubus idaeus
0.003
-
 4-coumaroyl-CoA pH 8.0, 30°C Rubus idaeus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
 2 * 41500, SDS-PAGE Rubus idaeus
83000
-
 gel filtration Rubus idaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus Royalty
-
 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Rubus idaeus
-
-
-
Rubus idaeus Royalty
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 172fold from fruits by ammonium sulfate fractionation, gel filtration, hydrophobic interaction and anion exchange chromatography, and chromatofocusing Rubus idaeus

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Rubus idaeus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
4-coumaroyl-CoA + malonyl-CoA + H2O high activity with 4-coumaroyl-CoA Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
4-coumaroyl-CoA + malonyl-CoA + H2O high activity with 4-coumaroyl-CoA Rubus idaeus Royalty 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
 ?
5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
caffeoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
caffeoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
cinnamoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
cinnamoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus Royalty 2 CoA + ? + 2 CO2
-
 ?
feruloyl-CoA + malonyl-CoA + H2O feruloyl-CoA is the best substrate showing 3fold higher activity than 4-coumaroyl-CoA Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?
sinapoyl-CoA + malonyl-CoA + H2O
-
 Rubus idaeus 2 CoA + ? + 2 CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41500, SDS-PAGE Rubus idaeus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rubus idaeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rubus idaeus

pI Value

Organism Comment pI Value Maximum pI Value
Rubus idaeus chromatofocusing
-
 5.3

Expression

Organism Comment Expression
Rubus idaeus rapid induction of the enzyme in cell suspension cultures upon addition of yeast extract up

General Information

General Information Comment Organism
physiological function 4-hydroxybenzalacetone, i.e. 4-hydroxyphenylbutan-2-one, the characteristic aroma compound of raspberries, is synthesized from 4-coumaryl-CoA and malonyl-CoA in a two-step reaction sequence that is catalyzed by benzalacetone synthase and benzalacetone reductase. 4-Hydroxybenzalacetone inhibits mycelial growth of the raspberry pathogen Phytophthora fragariae var. rub Rubus idaeus