Literature summary for 2.3.1.212 extracted from

Morita, H.; Tanio, M.; Kondo, S.; Kato, R.; Wanibuchi, K.; Noguchi, H.; Sugio, S.; Abe, I.; Kohno, T.
Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone (2008), Acta Crystallogr. Sect. F, 64, 304-306.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution	Rheum palmatum

Crystallization (Comment)

Organism

purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution

Rheum palmatum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	2 * 43000, SDS-PAGE	Rheum palmatum
83000	-	gel filtration	Rheum palmatum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O	Rheum palmatum	-	2 CoA + 4-hydroxybenzalacetone + 2 CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Rheum palmatum	Q94FV7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O	-	Rheum palmatum	2 CoA + 4-hydroxybenzalacetone + 2 CO2	-	?
4-coumaroyl-CoA + malonyl-CoA + H2O	one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one	Rheum palmatum	2 CoA + 4-hydroxybenzalacetone + 2 CO2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 43000, SDS-PAGE	Rheum palmatum
More	structure-function relationship, overview	Rheum palmatum

Synonyms

Synonyms	Comment	Organism
bAS	-	Rheum palmatum

General Information

General Information	Comment	Organism
evolution	the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily	Rheum palmatum
additional information	structure-function relationship, overview	Rheum palmatum