Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozyme CPT1A in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A478G | site-directed mutagenesis, the mutant shows decreased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
E26K | site-directed mutagenesis, the mutant shows decreased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
E26K/K561E | site-directed mutagenesis, the double mutant shows the same sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
K561E | site-directed mutagenesis, the mutant shows decreased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
M593S | site-directed mutagenesis, the mutant is insensitive to malonyl-CoA | Homo sapiens |
R243T | site-directed mutagenesis, the mutant shows highly decreased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
R243T/A478G | site-directed mutagenesis, the mutant shows highly decreased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | IC50: 0.0073 mM for the recombinant wild-type isozyme CPT1A, IC50: 0.0384 mM for CPT1A mutant R243T, IC50 values of other mutants, overview, determination of two binding sites, the A site and the O site, the latter involved residue Arg243, binding structure, interactions between N- and C-terminal residues are involved in malonyl-CoA binding to the A site, isozyme CPT1A in malonyl-CoA molecular docking, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
palmitoyl-CoA | 30°C, recombinant CPT1A mutant R243T | Homo sapiens | |
0.056 | - |
L-carnitine | 30°C, recombinant CPT1A mutant R243T | Homo sapiens | |
0.135 | - |
L-carnitine | 30°C, recombinant wildtype isozyme CPT1A | Homo sapiens | |
0.4 | - |
palmitoyl-CoA | 30°C, recombinant wildtype isozyme CPT1A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | Homo sapiens | - |
CoA + L-palmitoylcarnitine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme CPT1A | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | isozyme CPT1A | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + L-palmitoylcarnitine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carnitine palmitoyltransferase 1A | - |
Homo sapiens |
CPT1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics with malonyl-CoA | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0073 | 0.0384 | IC50: 0.0073 mM for the recombinant wild-type isozyme CPT1A, IC50: 0.0384 mM for CPT1A mutant R243T, IC50 values of other mutants, overview, determination of two binding sites, the A site and the O site, the latter involved residue Arg243, binding struct | Homo sapiens | malonyl-CoA |