Any feedback?
Literature summary for 2.3.1.21 extracted from
- Cysteine-scanning mutagenesis of muscle carnitine palmitoyltransferase I reveals a single cysteine residue (Cys-305) is important for catalysis (2005), J. Biol. Chem., 280, 4524-4531.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant isozymes M-CPT I in Pichia pastoris strain GS115 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A305C | site-directed mutagenesis, the revertant mutant shows about wild-type activity | Homo sapiens |
| C305A | site-directed mutagenesis, inactive mutant | Homo sapiens |
| C448A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C504A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C526A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C548S | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C562A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C586A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but reduced sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C608A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
| C659A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malonyl-CoA | IC50: 0.0005 mM for the recombinant wild-type enzyme, IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I, overview | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens | |
| 0.073 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.824 | - |
L-carnitine | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.92 | - |
L-carnitine | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-carnitine | Homo sapiens | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | CoA + L-palmitoylcarnitine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme M-CPT I | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine | the catalytic triad is composed of Cys305, His473, and Asp454, with Cys305 serving as a probable nucleophile, thus acting as a site for covalent attachment of the acyl molecule and formation of a stable acyl-enzyme intermediate, mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | isozyme M-CPT I | Homo sapiens | - |
| muscle | isozyme M-CPT I | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of mutant enzymes | Homo sapiens |
| 0.0028 | - |
recombinant revertant A305C | Homo sapiens |
| 0.0029 | - |
recombinant wild-type enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + L-palmitoylcarnitine | - |
? | |
| palmitoyl-CoA + L-carnitine | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | Homo sapiens | CoA + L-palmitoylcarnitine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| muscle carnitine palmitoyltransferase I | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
IC50: 0.0005 mM for the recombinant wild-type enzyme | Homo sapiens | malonyl-CoA | |
| 0.00052 | - |
IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I overview | Homo sapiens | malonyl-CoA | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
