Cloned (Comment) | Organism |
---|---|
expression of carnitine palmitoyltransferase I wild-type and mutant enzymes in Saccharomyces cerevisiae | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
A478G | 3.2fold increase in IC50 for malonyl-CoA, 2.6fold increase in KM-value for carnitine, 3.1fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
C608A | 2.2fold increase in IC50 for malonyl-CoA, 2.5fold increase in KM-value for carnitine, 5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
M593A | 12.6fold increase in IC50 for malonyl-CoA, 2.3fold increase in KM-value for carnitine, 1.2fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
M593E | 18fold increase in IC50 for malonyl-CoA, 1.2fold increase in KM-value for carnitine, 1.3fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
M593S | 26fold increase in IC50 for malonyl-CoA, KM-value for carnitine is nearly identical to wild-type value, 1.5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
N464D | 1.4fold decrease in IC50 for malonyl-CoA, 1.8fold increase in KM-value for carnitine, 1.2fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
T314S | 1.2fold increase in IC50 for malonyl-CoA, 1.4fold increase in KM-value for carnitine, 2.9fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | IC50: 0.0123 for wild-type enzyme, 0.015 mM for mutant enzyme T314S, 0.0087 mM for mutant enzyme N464D, 0.0395 mM for mutant enzyme A478G, 0.0275 mM for mutant enzyme C608A, 0.319 mM for mutant enzyme M593 mM, 0.155 mM for mutant enzyme M593A, 0.22 mM for mutant enzyme M593E | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
palmitoyl-CoA | mutant T314S | Rattus norvegicus | |
0.0017 | - |
L-carnitine | mutant T314S | Rattus norvegicus | |
0.0041 | - |
palmitoyl-CoA | mutant N464D | Rattus norvegicus | |
0.0041 | - |
L-carnitine | mutant N464D | Rattus norvegicus | |
0.0049 | - |
palmitoyl-CoA | wild-type | Rattus norvegicus | |
0.0049 | - |
L-carnitine | wild-type | Rattus norvegicus | |
0.0061 | - |
palmitoyl-CoA | mutant M593A | Rattus norvegicus | |
0.0061 | - |
L-carnitine | mutant M593A | Rattus norvegicus | |
0.0063 | - |
palmitoyl-CoA | mutant M593E | Rattus norvegicus | |
0.0063 | - |
L-carnitine | mutant M593E | Rattus norvegicus | |
0.0074 | - |
palmitoyl-CoA | mutant M593S | Rattus norvegicus | |
0.0074 | - |
L-carnitine | mutant M593S | Rattus norvegicus | |
0.0151 | - |
palmitoyl-CoA | mutant A478G | Rattus norvegicus | |
0.0151 | - |
L-carnitine | mutant A478G | Rattus norvegicus | |
0.0243 | - |
palmitoyl-CoA | mutant C608A | Rattus norvegicus | |
0.0243 | - |
L-carnitine | mutant C608A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P32198 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carnitine palmitoyltransferase I | - |
Rattus norvegicus |
CPT I | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0087 | - |
IC50 0.0087 mM for mutant enzyme N464D | Rattus norvegicus | malonyl-CoA | |
0.0123 | - |
IC50: 0.0123 for wild-type enzyme | Rattus norvegicus | malonyl-CoA | |
0.015 | - |
IC50 0.015 mM for mutant enzyme T314S | Rattus norvegicus | malonyl-CoA | |
0.0275 | - |
IC50 0.0275 mM for mutant enzyme C608A | Rattus norvegicus | malonyl-CoA | |
0.0395 | - |
IC50 0.0395 mM for mutant enzyme A478G | Rattus norvegicus | malonyl-CoA | |
0.155 | - |
IC50 0.155 mM for mutant enzyme M593A | Rattus norvegicus | malonyl-CoA | |
0.319 | - |
IC50 0.319 mM for mutant enzyme M593 mM | Rattus norvegicus | malonyl-CoA |