Literature summary for 2.3.1.206 extracted from

Kearsey, L.J.; Prandi, N.; Karuppiah, V.; Yan, C.; Leys, D.; Toogood, H.; Takano, E.; Scrutton, N.S.
Structure of the Cannabis sativa olivetol-producing enzyme reveals cyclization plasticity in type III polyketide synthases (2019), FEBS J., 287, 1511-1524 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Cannabis sativa

Crystallization (Commentary)

Crystallization (Comment)	Organism
cocrystallization of the enzyme with CoA (2 mM) is performed in 0.2 M potassium nitrate containing 20% w/v PEG 3350 for 72 h at 4 °C using the sitting drop vapour diffusion technique. Crystals are cryo-protected in the mother liquor containing 20% glycerol, followed by flash freezing in liquid nitrogen	Cannabis sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3 malonyl-CoA + hexanoyl-CoA	Cannabis sativa	-	3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Cannabis sativa	B1Q2B6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Cannabis sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 malonyl-CoA + hexanoyl-CoA	-	Cannabis sativa	3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2	-	?
3 malonyl-CoA + hexanoyl-CoA	in the absence of olivetolic acid cyclase (OAC), a nonenzymatic C2 -> C7 decarboxylative aldol condensation of the tetraketide intermediate occurs forming olivetol. The apparent plasticity of the enzyme with only minor alterations in amino acid sequences demonstrates the subtle nature of the mechanistic discrimination. This involves interplay between multiple residues, an inherently flexible active site designed to accommodate a growing scaffold and easily convertible cyclization mechanism(s) with minimal intervention	Cannabis sativa	3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2	-	?

Synonyms

Synonyms	Comment	Organism
tetraketide synthase	-	Cannabis sativa
TKS	-	Cannabis sativa

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Release 2023.1 (January 2023)