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Literature summary for 2.3.1.205 extracted from

  • Liu, X.; Wang, L.; Steffan, N.; Yin, W.; Li, S.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus: FgaAT catalyses the acetylation of fumigaclavine B (2009), ChemBioChem, 10, 2325-2328.
    View publication on PubMed
Search for more literature for 2.3.1.205

Cloned(Commentary)

Cloned (Comment) Organism
gene AFUA_2G18020, DNA and amino acid seqquence determmination and analysis, sequence comparison, expression of His6-tagged FgaAT in Escherichia coli Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the FgaAT reaction apparently follows Michaelis-Menten kinetics Aspergillus fumigatus
0.122
-
 acetyl-CoA pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus
0.147
-
 fumigaclavine B pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 22% activation at 5 mM Aspergillus fumigatus
additional information no effects by Na+ and K+ at 5 mM, the acetyl-transfer reaction catalysed by FgaAT is very likely independent of the presence of metal ions Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
56700
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
57000
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
60800
-
 recombinant His6-tagged enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + fumigaclavine B Aspergillus fumigatus
-
 CoA + fumigaclavine A
-
 ?
acetyl-CoA + fumigaclavine B Aspergillus fumigatus Af293
-
 CoA + fumigaclavine A
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WZ64 gene AFUA_2G18020
-
Aspergillus fumigatus Af293 Q4WZ64 gene AFUA_2G18020
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + fumigaclavine B
-
 Aspergillus fumigatus CoA + fumigaclavine A
-
 ?
acetyl-CoA + fumigaclavine B
-
 Aspergillus fumigatus Af293 CoA + fumigaclavine A
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
FgaAT
-
 Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 acetyl-CoA pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus
0.6
-
 fumigaclavine B pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function FgaAT catalyses the acetylation of fumigaclavine B, the conversion of fumigaclavine B to A, which belong to the ergot alkaloids of the clavine-type Aspergillus fumigatus