BRENDA - Enzyme Database show
show all sequences of 2.3.1.202

Structural analysis of PseH, the Campylobacter jejuni N-acetyltransferase involved in bacterial O-linked glycosylation

Song, W.S.; Nam, M.S.; Namgung, B.; Yoon, S.I.; Biochem. Biophys. Res. Commun. 458, 843-848 (2015)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
PseH alone and in complex with AcCoA, to 1.95 A resolution. PseH folds into a single-domain structure of a central beta-sheet decorated by four alpha-helices with two continuously connected grooves. A deep groove accommodates the AcCoA molecule. The acetyl end of AcCoA points toward an open space in a neighboring shallow groove, which is occupied by extra electron density that potentially serves as a pseudosubstrate. PseH may utilize a catalytic mechanism of acetylation different from other glycosylation-associated acetyltransferases
Campylobacter jejuni
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni
A0A0J9X276
subsp. jejuni
-
Campylobacter jejuni PT14
A0A0J9X276
subsp. jejuni
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
-
735607
Campylobacter jejuni
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
-
-
-
?
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
-
735607
Campylobacter jejuni PT14
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
-
-
-
?
Crystallization (Commentary) (protein specific)
Crystallization
Organism
PseH alone and in complex with AcCoA, to 1.95 A resolution. PseH folds into a single-domain structure of a central beta-sheet decorated by four alpha-helices with two continuously connected grooves. A deep groove accommodates the AcCoA molecule. The acetyl end of AcCoA points toward an open space in a neighboring shallow groove, which is occupied by extra electron density that potentially serves as a pseudosubstrate. PseH may utilize a catalytic mechanism of acetylation different from other glycosylation-associated acetyltransferases
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
-
735607
Campylobacter jejuni
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
-
-
-
?
acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
-
735607
Campylobacter jejuni PT14
CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
-
-
-
?
Other publictions for EC 2.3.1.202
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735607
Song
Structural analysis of PseH, t ...
Campylobacter jejuni, Campylobacter jejuni PT14
Biochem. Biophys. Res. Commun.
458
843-848
2015
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737066
Ud-Din
Crystal structure of Helicobac ...
Helicobacter pylori, Helicobacter pylori ATCC 700392
PLoS ONE
10
e0115634
2015
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735392
Liu
Cloning, purification and prel ...
Helicobacter pylori, Helicobacter pylori ATCC 700392
Acta Crystallogr. Sect. F
70
1276-1279
2014
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692521
Schoenhofen
Elucidation of the CMP-pseudam ...
Helicobacter pylori
Glycobiology
16
8C-14C
2006
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