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Literature summary for 2.3.1.20 extracted from

  • Elamin, A.A.; Stehr, M.; Spallek, R.; Rohde, M.; Singh, M.
    The Mycobacterium tuberculosis Ag85A is a novel diacylglycerol acyltransferase involved in lipid body formation (2011), Mol. Microbiol., 81, 1577-1592.
    View publication on PubMed
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Mycobacterium smegmatis Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
S126A no detectable activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 palmitoyl-CoA pH 7.0, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQP3
-
-
Mycobacterium tuberculosis H37Rv P9WQP3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
-
 Mycobacterium tuberculosis rac-tripalmitoylglycerol + CoA
-
 ?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
-
 Mycobacterium tuberculosis H37Rv rac-tripalmitoylglycerol + CoA
-
 ?
additional information enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate Mycobacterium tuberculosis ?
-
 ?
additional information enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
FBPA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.92
-
 palmitoyl-CoA pH 7.0, 37°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function upon overexpression in Mycobacterium smegmatis mc2155, cell morphology is changed and the cells become grossly enlarged. A massive formation of lipid bodies and a change in lipid pattern is observed simultaneously Mycobacterium tuberculosis