Literature summary for 2.3.1.20 extracted from

Xu, J.; Francis, T.; Mietkiewska, E.; Giblin, E.M.; Barton, D.L.; Zhang, Y.; Zhang, M.; Taylor, D.C.
Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content (2008), Plant Biotechnol. J., 6, 799-818.

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Application

Application	Comment	Organism
biotechnology	overexpression of mutated DGAT1 in Arabidopsis can be used to enhance oil content	Tropaeolum majus

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of recombinant TmDGAT1 in the yeast H1246MATalpha quadruple mutant (DGA1, LRO1, ARE1, ARE2) restores the capability of the mutant host to produce triacylglycerols	Tropaeolum majus
additional information	in plant transformation studies, seed-specific expression of TmDGAT1 is able to complement the low TAG/unusual fatty acid phenotype of the Arabidopsis AS11 (DGAT1) mutant	Tropaeolum majus
additional information	overexpression of TmDGAT1 in wild-type Arabidopsis and high-erucic-acid rapeseed (HEAR) and canola Brassica napus results in an increase in oil content (3.5%-10% on a dry weight basis, or a net increase of 11%-30%)	Tropaeolum majus
additional information	site-directed mutagenesis is conducted on six putative functional regions/motifs of the TmDGAT1 enzyme. Mutagenesis of a serine residue in a putative SnRK1 target site results in a 38%-80% increase in DGAT1 activity, and over-expression of the mutated TmDGAT1 in Arabidopsis results in a 20%-50% increase in oil content on a per seed basis	Tropaeolum majus

Organism

Organism	UniProt	Comment	Textmining
Tropaeolum majus	Q8RX96	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	gene is expressed exclusively in developing seeds	Tropaeolum majus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	recombinant TmDGAT1 protein is capable of utilizing a range of (14)C-labelled fatty acyl-CoA donors and diacylglycerol acceptors, and can synthesize (14)C-trierucin	Tropaeolum majus	?	-	?

Synonyms

Synonyms	Comment	Organism
diacylglycerol acyltransferase 1	-	Tropaeolum majus
TmDGAT1	-	Tropaeolum majus

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Release 2023.1 (January 2023)