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Literature summary for 2.3.1.20 extracted from
- Identification and functional expression of a type 2 acyl-CoA:diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals (2006), Phytochemistry, 67, 2541-2549.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DGAT1, DNA and amino acid sequence determination and analysis, expression analysis of DGAT1 and DGAT2, expression in Saccharomyces cerevisiae microsomes, phylogenetic analysis | Ricinus communis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Ricinus communis | 16020 | - |
| microsome | - |
Ricinus communis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Ricinus communis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA | Ricinus communis | - |
triricinolein + CoA | - |
? | |
| additional information | Ricinus communis | in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
cv. 99N89I, genes DGAT1 and DGAT2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | DGAT2 is 18fold more highly expressed in seeds than in leaves and shows temporal specific expression during seed development, while DGAT1 shows little dfference in expression in seeds versus leaves, overview | Ricinus communis | - |
| seed | DGAT2 is 18fold more highly expressed in seeds than in leaves and shows temporal specific expression during seed development, while DGAT1 shows little dfference in expression in seeds versus leaves, overview | Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quantitative expression analysis of DGAT1 and DGAT2 | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA | - |
Ricinus communis | triricinolein + CoA | - |
? | |
| additional information | in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1 | Ricinus communis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DGAT | - |
Ricinus communis |
| type 2 acyl-CoA:diacylglycerol acyltransferase | - |
Ricinus communis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ricinus communis |
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Release 2023.1 (January 2023)
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