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Literature summary for 2.3.1.20 extracted from
- Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleaginous fungus (1997), J. Biochem., 121, 1107-1114.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidic acid | - |
Umbelopsis ramanniana |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 2 mM, inhibits purified enzyme but not activity in lipid body fraction | Umbelopsis ramanniana | |
| KCl | 500 mM, inhibits purified enzyme | Umbelopsis ramanniana | |
| MgCl2 | 5 mM, inhibits purified enzyme but not activity in lipid body fraction | Umbelopsis ramanniana | |
| sodiumdodecyl sulfate | irreversible denaturation | Umbelopsis ramanniana | |
| sphingosine | - |
Umbelopsis ramanniana | |
| Trifluoperazine | 0.5 mM, in the presence of suboptimal phosphatidic acid concentration | Umbelopsis ramanniana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lipid storage body | - |
Umbelopsis ramanniana | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Umbelopsis ramanniana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Umbelopsis ramanniana | - |
var angulispora, fungus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4802fold | Umbelopsis ramanniana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
- |
Umbelopsis ramanniana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacylglycerol | - |
Umbelopsis ramanniana | CoA + triacylglycerol | - |
? | |
| acyl-CoA + 1,3-dioleoylglycerol | - |
Umbelopsis ramanniana | CoA + 2-acyl-1,3-dioleoylglycerol | - |
? | |
| acyl-CoA + lysophosphatidylcholine | - |
Umbelopsis ramanniana | CoA + acyllysophosphatidylcholine | - |
? | |
| acyl-CoA + rac-1,2-dioleoylglycerol | - |
Umbelopsis ramanniana | CoA + 3-acyl-rac-1,2-dioleoylglycerol | - |
? | |
| acyl-CoA + sn-1,2-diolein | - |
Umbelopsis ramanniana | CoA + acyl-sn-1,2-diolein | - |
? | |
| acyl-CoA + sn-2-monoolein | - |
Umbelopsis ramanniana | CoA + ? | - |
? | |
| lauroyl-CoA + 1,2-diacylglycerol | - |
Umbelopsis ramanniana | CoA + 1,2-diacyl-3-lauroylglycerol | - |
? | |
| oleoyl-CoA + 1,2-diacylglycerol | - |
Umbelopsis ramanniana | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
complete loss of activity after 30 min | Umbelopsis ramanniana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Umbelopsis ramanniana |
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