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Literature summary for 2.3.1.196 extracted from

  • Boatright, J.; Negre, F.; Chen, X.; Kish, C.M.; Wood, B.; Peel, G.; Orlova, I.; Gang, D.; Rhodes, D.; Dudareva, N.
    Understanding in vivo benzenoid metabolism in petunia petal tissue (2004), Plant Physiol., 135, 1993-2011.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: acetyl-CoA Petunia x hybrida
0.444
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
0.682
-
acetyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
0.686
-
2-phenylethanol pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida
0.875
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida
1.56
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51040
-
calculated from sequence Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + 2-phenylethanol Petunia x hybrida the enzyme is most likely responsible for the formation of both 2-phenylethyl benzoate and benzyl benzoate in vivo 2-phenylethyl benzoate + CoA
-
?
benzoyl-CoA + benzyl alcohol Petunia x hybrida the enzyme is most likely responsible for the formation of both 2-phenylethyl benzoate and benzyl benzoate in vivo benzyl benzoate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Petunia x hybrida Q6E593 cv Mitchell
-

Purification (Commentary)

Purification (Comment) Organism
-
Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining
corolla BPBT mRNA transcripts predominante in the limb of petunia corollas, the parts of the flower that are primarily responsible for scent production and emission in petunia. The steady-state BPBT mRNA level in corolla limbs is developmentally regulated, peaking 1 to 2 d after anthesis and changing rhythmically during a daily light/dark cycle closely correlating with the pattern of BPBT activity and benzylbenzoate accumulation Petunia x hybrida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + benzyl alcohol turnover number for acetyl is 5-8% of that with benzoyl-CoA Petunia x hybrida benzyl acetate + CoA
-
?
benzoyl-CoA + 2-phenylethanol
-
Petunia x hybrida 2-phenylethyl benzoate + CoA
-
?
benzoyl-CoA + 2-phenylethanol the enzyme is most likely responsible for the formation of both 2-phenylethyl benzoate and benzyl benzoate in vivo Petunia x hybrida 2-phenylethyl benzoate + CoA
-
?
benzoyl-CoA + benzyl alcohol
-
Petunia x hybrida benzyl benzoate + CoA
-
?
benzoyl-CoA + benzyl alcohol the enzyme is most likely responsible for the formation of both 2-phenylethyl benzoate and benzyl benzoate in vivo Petunia x hybrida benzyl benzoate + CoA
-
?

Synonyms

Synonyms Comment Organism
BEBT1
-
Petunia x hybrida
benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase
-
Petunia x hybrida
BPBT
-
Petunia x hybrida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Petunia x hybrida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.61
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: acetyl-CoA Petunia x hybrida
4.4
-
acetyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
35.8
-
2-phenylethanol pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida
36.9
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
57.1
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
81.3
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Petunia x hybrida

pI Value

Organism Comment pI Value Maximum pI Value
Petunia x hybrida calculated from sequence
-
6.12

General Information

General Information Comment Organism
physiological function the enzyme involved in benzenoid metabolism Petunia x hybrida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.68
-
acetyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
37.2
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
52.8
-
2-phenylethanol pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida
56.8
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: acetyl-CoA Petunia x hybrida
83.1
-
benzyl alcohol pH 7.7, 22°C, cosubstrate: benzyl alcohol Petunia x hybrida
92.2
-
benzoyl-CoA pH 7.7, 22°C, cosubstrate: 2-phenylethanol Petunia x hybrida