Cloned (Comment) | Organism |
---|---|
gene nphT7, functional recombinant expression in sugarcane (Saccharum sp.) cultivar Q117, coexpression of gene nphT7 with genes phaB and phaC | Streptomyces sp. CL190 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering the production of polyhydroxyalkanoates (PHAs) into high biomass bioenergy crops has the potential to provide a sustainable supply of bioplastics and energy from a single plant feedstock. One of the major challenges in engineering C4 plants for the production of poly[(R)-3-hydroxybutyrate] (PHB) is the significantly lower level of polymer produced in the chloroplasts of mesophyll cells compared to bundle sheath cells, thereby limiting the full PHB yield potential of the plant. The access to substrate for PHB synthesis may limit polymer production in mesophyll cell chloroplasts. The use of an acetoacetyl-CoA synthase, that catalyses the conversion of acetyl-CoA and malonyl-CoA to acetoacetyl-CoA with the release of carbon dioxide, in place of a beta-ketothiolase, the first enzyme in the bacterial PHA pathway, enhances poly-3-hydroxybutyrate production in sugarcane mesophyll cells. The engineered cells shows increased production of PHB and increased polymer molecular weight. PhaA is the main contributor to low PHB yield in mesophyll cells. Phenotypes of transgenic sugarcane leafs, overview | Streptomyces sp. CL190 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-CoA | Streptomyces sp. CL190 | - |
acetoacetyl-CoA + CoA + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. CL190 | D7URV0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-CoA | - |
Streptomyces sp. CL190 | acetoacetyl-CoA + CoA + CO2 | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
AACS | - |
Streptomyces sp. CL190 |
Acetoacetyl-CoA synthase | - |
Streptomyces sp. CL190 |
NphT7 | - |
Streptomyces sp. CL190 |