Literature summary for 2.3.1.194 extracted from

Tippmann, S.; Ferreira, R.; Siewers, V.; Nielsen, J.; Chen, Y.
Effects of acetoacetyl-CoA synthase expression on production of farnesene in Saccharomyces cerevisiae (2017), J. Ind. Microbiol. Biotechnol., 44, 911-922 .

Search for more literature for 2.3.1.194

Application

Application	Comment	Organism
biotechnology	the enzyme represents a potential target to increase the flux through the mevalonate pathway	Streptomyces sp. CL190
biotechnology	the enzyme represents a potential target to increase the flux through the mevalonate pathway	Streptomyces glaucescens

Cloned(Commentary)

Cloned (Comment)	Organism
gene nphT7, recombinant expression in Saccharomyces cerevisiae strain CEN, analysis of the effect of acetoacetyl-CoA synthase on growth as well as the production of farnesene and compares different homologues regarding their efficiency	Streptomyces sp. CL190

Protein Variants

Protein Variants	Comment	Organism
additional information	heterologous expression of different nphT7 homologues for farnesene production. acetoacetyl-CoA synthase from might increase the flux through the mevalonate pathway, effect of acetoacetyl-CoA synthase on growth as well as the production of farnesene, overview. While plasmid-based expression of nphT7 does not improve final farnesene titers, the construction of an alternative pathway, which exclusively relies on the malonyl-CoA bypass, was detrimental for growth and farnesene production. The overall functionality of the bypass is limited by the efficiency of acetoacetyl-CoA synthase. Gene nphT7 from Streptomyces glaucescens shows clearly higher efficiency compared to Streptomyces sp. strain CL190, overview	Streptomyces sp. CL190
additional information	heterologous expression of different nphT7 homologues for farnesene production. acetoacetyl-CoA synthase might increase the flux through the mevalonate pathway, effect of acetoacetyl-CoA synthase on growth as well as the production of farnesene, overview. While plasmid-based expression of nphT7 does not improve final farnesene titers, the construction of an alternative pathway, which exclusively relies on the malonyl-CoA bypass, was detrimental for growth and farnesene production. The overall functionality of the bypass is limited by the efficiency of acetoacetyl-CoA synthase. Gene nphT7 from Streptomyces glaucescens shows clearly higher efficiency compared to Streptomyces sp. strain CL190, overview	Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + malonyl-CoA	Streptomyces sp. CL190	-	acetoacetyl-CoA + CoA + CO2	-	ir
acetyl-CoA + malonyl-CoA	Streptomyces glaucescens	-	acetoacetyl-CoA + CoA + CO2	-	ir

Organism

Organism	UniProt	Comment	Textmining
Streptomyces glaucescens	A0A089XE59	-	-
Streptomyces sp. CL190	D7URV0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + malonyl-CoA	-	Streptomyces sp. CL190	acetoacetyl-CoA + CoA + CO2	-	ir
acetyl-CoA + malonyl-CoA	-	Streptomyces glaucescens	acetoacetyl-CoA + CoA + CO2	-	ir

Synonyms

Synonyms	Comment	Organism
Acetoacetyl-CoA synthase	-	Streptomyces sp. CL190
Acetoacetyl-CoA synthase	-	Streptomyces glaucescens
NphT7	-	Streptomyces sp. CL190
NphT7	-	Streptomyces glaucescens
nphT7SCL	-	Streptomyces sp. CL190

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Release 2023.1 (January 2023)