BRENDA - Enzyme Database show
show all sequences of 2.3.1.192

Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization

Nandi, D.L.; Lucas, S.V.; Webster, L.T.; J. Biol. Chem. 254, 7230-7237 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation
Bos taurus
acylglycine
noncompetitive
Bos taurus
benzoyl-CoA
-
Bos taurus
butyryl-CoA
-
Bos taurus
CoA
noncompetitve
Bos taurus
Ni2+
2 mM, 28% residual activity
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
Zn2+
1 mM, 46% residual activity
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
addition of monovalent cations is required
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Reaction
Reaction
Commentary
Organism
phenylacetyl-CoA + glycine = CoA + phenylacetylglycine
sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves, and the peptide product dissociates last
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.051
-
pH not specified in the publication, temperature not specified in the publication
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indoleacetyl-CoA + glycine
-
486265
Bos taurus
N-indoleacetylglycine + CoA
-
-
-
?
phenylacetyl-CoA + glycine
glycine is the preferred acceptor
486265
Bos taurus
phenylacetylglycine + CoA
-
-
-
?
phenylacetyl-CoA + L-asparagine
stereospecific for the L-isomer of asparagine
486265
Bos taurus
? + CoA
-
-
-
?
phenylacetyl-CoA + L-glutamine
stereospecific for the L-isomer of glutamine
486265
Bos taurus
? + CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
8.6
-
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation
Bos taurus
acylglycine
noncompetitive
Bos taurus
benzoyl-CoA
-
Bos taurus
butyryl-CoA
-
Bos taurus
CoA
noncompetitve
Bos taurus
Ni2+
2 mM, 28% residual activity
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
Zn2+
1 mM, 46% residual activity
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
addition of monovalent cations is required
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.051
-
pH not specified in the publication, temperature not specified in the publication
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indoleacetyl-CoA + glycine
-
486265
Bos taurus
N-indoleacetylglycine + CoA
-
-
-
?
phenylacetyl-CoA + glycine
glycine is the preferred acceptor
486265
Bos taurus
phenylacetylglycine + CoA
-
-
-
?
phenylacetyl-CoA + L-asparagine
stereospecific for the L-isomer of asparagine
486265
Bos taurus
? + CoA
-
-
-
?
phenylacetyl-CoA + L-glutamine
stereospecific for the L-isomer of glutamine
486265
Bos taurus
? + CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
8.6
-
Bos taurus
Other publictions for EC 2.3.1.192
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
708944
Vessey
Determination of the sequence ...
Bos taurus
J. Biochem. Mol. Toxicol.
12
275-279
1998
-
-
1
-
-
-
-
-
2
-
1
-
-
2
-
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487441
Kelley
Characterization of the acyl-C ...
Homo sapiens
J. Biochem. Toxicol.
9
153-158
1994
-
-
-
-
-
-
2
1
1
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487438
Kelley
The effects of ions on the con ...
Bos taurus
J. Biochem. Toxicol.
5
125-135
1990
2
-
-
-
-
-
5
-
1
3
2
-
-
1
-
-
1
-
-
1
1
-
5
1
-
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
1
5
-
-
1
3
2
-
-
-
-
1
-
1
1
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
707446
Kelley
Interaction of 2,4-dichlorophe ...
Bos taurus
Biochem. Pharmacol.
35
289-295
1986
-
-
-
-
-
-
2
2
1
-
-
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486265
Nandi
Benzoyl-coenzyme A:glycine N-a ...
Bos taurus
J. Biol. Chem.
254
7230-7237
1979
-
-
-
-
-
-
8
-
-
1
1
-
-
2
-
-
1
1
-
1
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
1
-
-
-
-
1
-
1
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-