BRENDA - Enzyme Database show
show all sequences of 2.3.1.191

A continuous fluorescent enzyme assay for early steps of lipid A biosynthesis

Jenkins, R.J.; Dotson, G.D.; Anal. Biochem. 425, 21-27 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
LpxD is a viable target for antimicrobial development
Escherichia coli
Cloned(Commentary)
Commentary
Organism
gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3)
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
0.012
UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
pH 8.0, 25C, recombinant His6-tagged LpxD
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
Escherichia coli
-
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein]
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
gene lpxD
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged LpxD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
-
718571
Escherichia coli
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein]
-
-
-
ir
additional information
fluorescent enzyme assay, method optimization, overview
718571
Escherichia coli
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
-
UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
pH 8.0, 25C, recombinant His6-tagged LpxD
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
drug development
LpxD is a viable target for antimicrobial development
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3)
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
0.012
UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
pH 8.0, 25C, recombinant His6-tagged LpxD
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
Escherichia coli
-
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein]
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged LpxD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
-
718571
Escherichia coli
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein]
-
-
-
ir
additional information
fluorescent enzyme assay, method optimization, overview
718571
Escherichia coli
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
-
UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
pH 8.0, 25C, recombinant His6-tagged LpxD
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
metabolism
LpxD catalyzes the third step of lipid A biosynthesis, an acyl-acyl carrier protein (ACP)-dependent transfer of a fatty acyl moiety to a UDP-glucosamine core ring, overview
Escherichia coli
physiological function
LpxD is essential for survival in Gram-negative bacteria
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
metabolism
LpxD catalyzes the third step of lipid A biosynthesis, an acyl-acyl carrier protein (ACP)-dependent transfer of a fatty acyl moiety to a UDP-glucosamine core ring, overview
Escherichia coli
physiological function
LpxD is essential for survival in Gram-negative bacteria
Escherichia coli
Other publictions for EC 2.3.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735568
Alshalchi
Expression of the lipopolysacc ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Arch. Microbiol.
197
135-145
2015
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1
1
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736235
Eshghi
Leptospira interrogans lpxD ho ...
Leptospira interrogans, Leptospira interrogans L495
Infect. Immun.
83
4314-4321
2015
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8
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1
1
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736449
Jenkins
Structural basis for the recog ...
Escherichia coli
J. Biol. Chem.
289
15527-15535
2014
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736880
Masoudi
Chasing acyl carrier protein t ...
Escherichia coli
Nature
505
422-426
2014
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1
1
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1
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737072
Emiola
A complete pathway model for l ...
Escherichia coli
PLoS ONE
10
e0121216
2014
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735384
Badger
Structure determination of Lpx ...
Acinetobacter baumannii, Acinetobacter baumannii SDF
Acta Crystallogr. Sect. F
69
6-9
2013
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1
1
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736185
Yeom
Pleiotropic effects of acyltra ...
Pseudomonas aeruginosa
Genes Cells
18
682-693
2013
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718571
Jenkins
A continuous fluorescent enzym ...
Escherichia coli
Anal. Biochem.
425
21-27
2012
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2
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720944
Li
LPS remodeling is an evolved s ...
Francisella tularensis subsp. novicida, Francisella tularensis subsp. novicida U112
Proc. Natl. Acad. Sci. USA
109
8716-8721
2012
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4
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3
3
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718513
Badger
The structure of LpxD from Pse ...
Pseudomonas aeruginosa
Acta Crystallogr. Sect. F
67
749-752
2011
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1
1
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720913
Li
Pathway for lipid A biosynthes ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Proc. Natl. Acad. Sci. USA
108
11387-11392
2011
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696356
Bartling
Crystal structure and acyl cha ...
Escherichia coli
Biochemistry
48
8672-8683
2009
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696264
Bartling
Steady-state kinetics and mech ...
Escherichia coli
Biochemistry
47
5290-5302
2008
1
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1
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10
-
8
21
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3
1
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1
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3
1
1
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23
1
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1
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10
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2
8
7
21
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3
1
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1
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1
1
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23
1
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11
11
698587
Bainbridge
Acyl chain specificity of the ...
Escherichia coli, Porphyromonas gingivalis
J. Bacteriol.
190
4549-4558
2008
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2
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694027
Albers
Expression of Legionella pneum ...
Legionella pneumophila
Microbiology
153
3817-3829
2007
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700935
Buetow
Structure and reactivity of Lp ...
Chlamydia trachomatis
Proc. Natl. Acad. Sci. USA
104
4321-4326
2007
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695627
Vaara
Outer membrane permeability ba ...
Escherichia coli
Antimicrob. Agents Chemother.
43
1459-1462
1999
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698088
Steeghs
Isolation and characterization ...
Neisseria meningitidis
Gene
190
263-270
1997
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697921
Vuorio
The novel hexapeptide motif fo ...
Yersinia enterocolitica
FEBS Lett.
337
289-292
1994
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698693
Kelly
Raetz, C.R.; Anderson, M.S.: T ...
Escherichia coli
J. Biol. Chem.
268
19866-19874
1993
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