BRENDA - Enzyme Database show
show all sequences of 2.3.1.191

The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution

Badger, J.; Chie-Leon, B.; Logan, C.; Sridhar, V.; Sankaran, B.; Zwart, P.H.; Nienaber, V.; Acta Crystallogr. Sect. F 67, 749-752 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)
Pseudomonas aeruginosa
Crystallization (Commentary)
Crystallization
Organism
purified recombinant His6-tagged LpxD, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml LpxD in 10 mM Tris pH 8, 500 mM NaCl and 1 mM DTT, with 50 mM uridine diphosphate-N-acetylglucosamine, is mixed with 0.002 ml of 100 mM Tris pH 8.5, 1.5 M lithium sulfate, 20°C, 3-5 days, X-ray diffraction structure determination and analysis at 1.3 A resolution, molecular replacement
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
Q9HX46
-
-
Purification (Commentary)
Commentary
Organism
recombinant N-terminally His6-tagged LpxD from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
Pseudomonas aeruginosa
Subunits
Subunits
Commentary
Organism
More
the LpxD domain architecture is consisting of an N-terminal domain, amino acids 2-95, a left-handed beta-helix central domain containing many short parallel beta-sheet segments wrappingthrough consecutive triangular sections, amino acids 101-305, and a C-terminal helix, amino acids 312-340
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant His6-tagged LpxD, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml LpxD in 10 mM Tris pH 8, 500 mM NaCl and 1 mM DTT, with 50 mM uridine diphosphate-N-acetylglucosamine, is mixed with 0.002 ml of 100 mM Tris pH 8.5, 1.5 M lithium sulfate, 20°C, 3-5 days, X-ray diffraction structure determination and analysis at 1.3 A resolution, molecular replacement
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged LpxD from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
Pseudomonas aeruginosa
Subunits (protein specific)
Subunits
Commentary
Organism
More
the LpxD domain architecture is consisting of an N-terminal domain, amino acids 2-95, a left-handed beta-helix central domain containing many short parallel beta-sheet segments wrappingthrough consecutive triangular sections, amino acids 101-305, and a C-terminal helix, amino acids 312-340
Pseudomonas aeruginosa
General Information
General Information
Commentary
Organism
physiological function
LpxD is part of the biosynthesis pathway of lipid A and is responsible for transferring 3-hydroxymyristic acid from the R-3-hydroxymyristoyl-acyl carrier protein to the 2-OH group of UDP-3-O-(3-hydroxymyristoyl) glucosamine. The first three enzymes responsible for Gram-negative bacterial cell-wall synthesis, LpxA, LpxC and LpxD, are all present as single copies and are essential for bacterial viability
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
physiological function
LpxD is part of the biosynthesis pathway of lipid A and is responsible for transferring 3-hydroxymyristic acid from the R-3-hydroxymyristoyl-acyl carrier protein to the 2-OH group of UDP-3-O-(3-hydroxymyristoyl) glucosamine. The first three enzymes responsible for Gram-negative bacterial cell-wall synthesis, LpxA, LpxC and LpxD, are all present as single copies and are essential for bacterial viability
Pseudomonas aeruginosa
Other publictions for EC 2.3.1.191
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2014
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PLoS ONE
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Structure determination of Lpx ...
Acinetobacter baumannii, Acinetobacter baumannii SDF
Acta Crystallogr. Sect. F
69
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2013
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3
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718513
Badger
The structure of LpxD from Pse ...
Pseudomonas aeruginosa
Acta Crystallogr. Sect. F
67
749-752
2011
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Pathway for lipid A biosynthes ...
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Steady-state kinetics and mech ...
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47
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2008
1
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1
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7
21
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1
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11
11
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Acyl chain specificity of the ...
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2008
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Albers
Expression of Legionella pneum ...
Legionella pneumophila
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2007
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Structure and reactivity of Lp ...
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Raetz, C.R.; Anderson, M.S.: T ...
Escherichia coli
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268
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