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show all sequences of 2.3.1.190

R-acetoin accumulation and dissimilation in Klebsiella pneumoniae

Wang, D.; Zhou, J.; Chen, C.; Wei, D.; Shi, J.; Jiang, B.; Liu, P.; Hao, J.; J. Ind. Microbiol. Biotechnol. 42, 1105-1115 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
engineering a budC acoABCD double mutant, which has lost 2,3-butanediol dehydrogenase activity and the acetoin dehydrogenase system for R-acetoin production. Optimized conditions include aerobic batch culture and mildly acidic conditions. 62.3 g per l R-acetoin can be produced by budC and acoABCD double mutants in 57 h culture, with an optical purity of 98.0%, and a substrate conversion ratio of 28.7%
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
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Klebsiella pneumoniae CGMCC 1.6366
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-
Application (protein specific)
Application
Commentary
Organism
synthesis
engineering a budC acoABCD double mutant, which has lost 2,3-butanediol dehydrogenase activity and the acetoin dehydrogenase system for R-acetoin production. Optimized conditions include aerobic batch culture and mildly acidic conditions. 62.3 g per l R-acetoin can be produced by budC and acoABCD double mutants in 57 h culture, with an optical purity of 98.0%, and a substrate conversion ratio of 28.7%
Klebsiella pneumoniae
General Information
General Information
Commentary
Organism
physiological function
the acetoin dehydrogenase enzyme system is responsible for R-acetoin dissimilation. Mutants lose the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated cannot be dissimilated. In the presence of another carbon source, the acetoin accumulated in broth of acetoin dehydrogenase mutants is converted to 2,3-butanediol
Klebsiella pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the acetoin dehydrogenase enzyme system is responsible for R-acetoin dissimilation. Mutants lose the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated cannot be dissimilated. In the presence of another carbon source, the acetoin accumulated in broth of acetoin dehydrogenase mutants is converted to 2,3-butanediol
Klebsiella pneumoniae
Other publictions for EC 2.3.1.190
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736592
Wang
R-acetoin accumulation and dis ...
Klebsiella pneumoniae, Klebsiella pneumoniae CGMCC 1.6366
J. Ind. Microbiol. Biotechnol.
42
1105-1115
2015
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703733
Payne
Discovery of a putative acetoi ...
Saccharolobus solfataricus
FEBS Lett.
584
1231-1234
2010
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704260
Huang
Biochemical and molecular char ...
Bacillus subtilis
J. Bacteriol.
181
3837-3841
1999
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Huang
Molecular characterization of ...
Pseudomonas putida
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141-150
1994
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704250
Krueger
Biochemical and molecular char ...
Clostridium magnum
J. Bacteriol.
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3614-3630
1994
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6
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701753
Lorenzl
Purification and characterizat ...
Clostridium magnum
Antonie van Leeuwenhoek
64
9-15
1993
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486168
Oppermann
Purification and characterizat ...
Pelobacter carbinolicus
J. Bacteriol.
173
757-767
1991
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704245
Priefert
Identification and molecular c ...
Alcaligenes eutrophus H16
J. Bacteriol.
173
4056-4071
1991
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