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show all sequences of 2.3.1.190

Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism

Priefert, H.; Hein, S.; Krueger, N.; Zeh, K.; Schmidt, B.; Steinbuechel, A.; J. Bacteriol. 173, 4056-4071 (1991)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
136000
-
gel filtration; gel filtration
Alcaligenes eutrophus H16
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alcaligenes eutrophus H16
P27745
subunit acoA
-
Alcaligenes eutrophus H16
P27746
subunit acoB
-
Purification (Commentary)
Commentary
Organism
from acetoin-grown cells; from acetoin-grown cells
Alcaligenes eutrophus H16
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.15
-
;
Alcaligenes eutrophus H16
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoin + 2,6-dichlorophenolindophenol
E1 complex
704245
Alcaligenes eutrophus H16
acetaldehyde + ?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 35243 Da and beta, 35788 Da, calculated and SDS-PAGE; component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 35243 Da and beta, 35788 Da, calculated and SDS-PAGE
Alcaligenes eutrophus H16
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
136000
-
gel filtration
Alcaligenes eutrophus H16
Purification (Commentary) (protein specific)
Commentary
Organism
from acetoin-grown cells
Alcaligenes eutrophus H16
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.15
-
-
Alcaligenes eutrophus H16
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoin + 2,6-dichlorophenolindophenol
E1 complex
704245
Alcaligenes eutrophus H16
acetaldehyde + ?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 35243 Da and beta, 35788 Da, calculated and SDS-PAGE
Alcaligenes eutrophus H16
Other publictions for EC 2.3.1.190
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736592
Wang
R-acetoin accumulation and dis ...
Klebsiella pneumoniae, Klebsiella pneumoniae CGMCC 1.6366
J. Ind. Microbiol. Biotechnol.
42
1105-1115
2015
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1
1
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703733
Payne
Discovery of a putative acetoi ...
Saccharolobus solfataricus
FEBS Lett.
584
1231-1234
2010
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1
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1
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1
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7
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8
1
1
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8
2
2
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704260
Huang
Biochemical and molecular char ...
Bacillus subtilis
J. Bacteriol.
181
3837-3841
1999
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-
1
-
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-
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4
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1
1
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703742
Huang
Molecular characterization of ...
Pseudomonas putida
FEMS Microbiol. Lett.
124
141-150
1994
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-
-
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2
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8
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1
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2
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704250
Krueger
Biochemical and molecular char ...
Clostridium magnum
J. Bacteriol.
176
3614-3630
1994
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1
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2
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6
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1
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1
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2
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2
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1
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2
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701753
Lorenzl
Purification and characterizat ...
Clostridium magnum
Antonie van Leeuwenhoek
64
9-15
1993
-
-
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-
-
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1
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4
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1
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1
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1
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486168
Oppermann
Purification and characterizat ...
Pelobacter carbinolicus
J. Bacteriol.
173
757-767
1991
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-
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1
1
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8
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1
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4
1
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1
1
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1
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4
1
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1
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1
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704245
Priefert
Identification and molecular c ...
Alcaligenes eutrophus H16
J. Bacteriol.
173
4056-4071
1991
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1
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4
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1
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1
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2
1
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2
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2
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