BRENDA - Enzyme Database show
show all sequences of 2.3.1.190

Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

Payne, K.A.; Hough, D.W.; Danson, M.J.; FEBS Lett. 584, 1231-1234 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
component E1alpha; component E1beta
Saccharolobus solfataricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0028
-
acetoin
E1 complex, 80C, pH 7.0; E1 complex, 80C, pH 7.0
Saccharolobus solfataricus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
142000
-
gel filtration, E1 complex; gel filtration, E1 complex
Saccharolobus solfataricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharolobus solfataricus
Q97YF5
E1beta subunit
-
Saccharolobus solfataricus
Q97YF6
E1alpha subunit
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoin + 2,6-dichlorophenolindophenol
E1 complex
703733
Saccharolobus solfataricus
acetaldehyde + ?
-
-
-
?
diacetyl + 2,6-dichlorophenolindophenol
E1 complex, 59% of the activity with acetoin
703733
Saccharolobus solfataricus
?
-
-
-
?
methyl acetoin + 2,6-dichlorophenolindophenol
E1 complex, 107% of the activity with acetoin
703733
Saccharolobus solfataricus
? + ?
-
-
-
?
additional information
no detectable activity with 2-oxoglutarate, pyruvate or the branched-chain 2-oxoacids, 4-methyl-2-oxopentanoate, 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate
703733
Saccharolobus solfataricus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 43000 and beta, 33000 Da, SDS-PAGE; component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 43000 and beta, 33000 Da, SDS-PAGE
Saccharolobus solfataricus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
80
85
;
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
Commentary
Organism
component E1alpha
Saccharolobus solfataricus
component E1beta
Saccharolobus solfataricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0028
-
acetoin
E1 complex, 80C, pH 7.0
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
142000
-
gel filtration, E1 complex
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoin + 2,6-dichlorophenolindophenol
E1 complex
703733
Saccharolobus solfataricus
acetaldehyde + ?
-
-
-
?
diacetyl + 2,6-dichlorophenolindophenol
E1 complex, 59% of the activity with acetoin
703733
Saccharolobus solfataricus
?
-
-
-
?
methyl acetoin + 2,6-dichlorophenolindophenol
E1 complex, 107% of the activity with acetoin
703733
Saccharolobus solfataricus
? + ?
-
-
-
?
additional information
no detectable activity with 2-oxoglutarate, pyruvate or the branched-chain 2-oxoacids, 4-methyl-2-oxopentanoate, 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate
703733
Saccharolobus solfataricus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 43000 and beta, 33000 Da, SDS-PAGE
Saccharolobus solfataricus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
80
85
-
Saccharolobus solfataricus
Other publictions for EC 2.3.1.190
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736592
Wang
R-acetoin accumulation and dis ...
Klebsiella pneumoniae, Klebsiella pneumoniae CGMCC 1.6366
J. Ind. Microbiol. Biotechnol.
42
1105-1115
2015
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1
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3
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1
1
-
-
-
703733
Payne
Discovery of a putative acetoi ...
Saccharolobus solfataricus
FEBS Lett.
584
1231-1234
2010
-
-
1
-
-
-
-
1
-
-
1
-
-
7
-
-
-
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-
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8
1
1
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2
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2
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2
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8
2
2
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704260
Huang
Biochemical and molecular char ...
Bacillus subtilis
J. Bacteriol.
181
3837-3841
1999
-
-
1
-
-
-
-
-
-
-
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4
-
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1
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1
1
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1
1
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703742
Huang
Molecular characterization of ...
Pseudomonas putida
FEMS Microbiol. Lett.
124
141-150
1994
-
-
-
-
-
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2
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8
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1
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2
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2
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704250
Krueger
Biochemical and molecular char ...
Clostridium magnum
J. Bacteriol.
176
3614-3630
1994
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1
-
-
-
-
-
-
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2
-
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6
-
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1
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1
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2
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2
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1
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2
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-
701753
Lorenzl
Purification and characterizat ...
Clostridium magnum
Antonie van Leeuwenhoek
64
9-15
1993
-
-
-
-
-
-
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1
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4
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1
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1
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1
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1
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1
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1
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1
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1
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486168
Oppermann
Purification and characterizat ...
Pelobacter carbinolicus
J. Bacteriol.
173
757-767
1991
-
-
-
-
-
-
-
-
-
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1
1
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8
-
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1
-
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4
1
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1
1
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1
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4
1
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-
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1
-
-
1
-
-
704245
Priefert
Identification and molecular c ...
Alcaligenes eutrophus H16
J. Bacteriol.
173
4056-4071
1991
-
-
-
-
-
-
-
-
-
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1
-
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4
-
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1
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1
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2
1
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2
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2
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2
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2
2
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