BRENDA - Enzyme Database show
show all sequences of 2.3.1.19

Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and its role in acidogenesis

Wiesenborn, D.P.; Rudolph, F.B.; Papoutsakis, E.T.; Appl. Environ. Microbiol. 55, 317-322 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Clostridium acetobutylicum
AMP
-
Clostridium acetobutylicum
ATP
-
Clostridium acetobutylicum
NADP+
-
Clostridium acetobutylicum
NADPH
-
Clostridium acetobutylicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.077
-
CoA
-
Clostridium acetobutylicum
0.11
-
butanoyl-CoA
-
Clostridium acetobutylicum
0.14
-
phosphate
-
Clostridium acetobutylicum
0.26
-
Butanoylphosphate
-
Clostridium acetobutylicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
8 * 31000, SDS-PAGE
Clostridium acetobutylicum
264000
-
gel filtration
Clostridium acetobutylicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium acetobutylicum
-
strain ATCC 824
-
Purification (Commentary)
Commentary
Organism
-
Clostridium acetobutylicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1380
-
-
Clostridium acetobutylicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + phosphate
1.3% of butanoylphosphate formation
486470
Clostridium acetobutylicum
acetylphosphate + CoA
-
-
-
?
butanoyl-CoA + phosphate
-
486470
Clostridium acetobutylicum
butanoylphosphate + CoA
-
486470
Clostridium acetobutylicum
?
isobutanoyl-CoA + phosphate
30% of butanoylphosphate formation
486470
Clostridium acetobutylicum
isobutanoylphosphate + CoA
-
-
-
?
isopentanoyl-CoA + phosphate
95% of butanoylphosphate formation
486470
Clostridium acetobutylicum
isopentanoylphosphate + CoA
-
-
-
?
n-pentanoyl-CoA + phosphate
78% of butanoylphosphate formation
486470
Clostridium acetobutylicum
n-pentanoylphosphate + CoA
-
-
-
?
n-propionyl-CoA + phosphate
23% of butanoylphosphate formation
486470
Clostridium acetobutylicum
n-propionylphosphate + CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
octamer
8 * 31000, SDS-PAGE
Clostridium acetobutylicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
butanoylphosphate formation
Clostridium acetobutylicum
8.8
-
butanoyl-CoA formation
Clostridium acetobutylicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
9.5
butanoylphosphate formation, less than 50% of maximal activity above and below
Clostridium acetobutylicum
6.8
9.5
butanoyl-CoA formation, less than 50% of maximal activity above and below
Clostridium acetobutylicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Clostridium acetobutylicum
AMP
-
Clostridium acetobutylicum
ATP
-
Clostridium acetobutylicum
NADP+
-
Clostridium acetobutylicum
NADPH
-
Clostridium acetobutylicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.077
-
CoA
-
Clostridium acetobutylicum
0.11
-
butanoyl-CoA
-
Clostridium acetobutylicum
0.14
-
phosphate
-
Clostridium acetobutylicum
0.26
-
Butanoylphosphate
-
Clostridium acetobutylicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
8 * 31000, SDS-PAGE
Clostridium acetobutylicum
264000
-
gel filtration
Clostridium acetobutylicum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium acetobutylicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1380
-
-
Clostridium acetobutylicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + phosphate
1.3% of butanoylphosphate formation
486470
Clostridium acetobutylicum
acetylphosphate + CoA
-
-
-
?
butanoyl-CoA + phosphate
-
486470
Clostridium acetobutylicum
butanoylphosphate + CoA
-
486470
Clostridium acetobutylicum
?
isobutanoyl-CoA + phosphate
30% of butanoylphosphate formation
486470
Clostridium acetobutylicum
isobutanoylphosphate + CoA
-
-
-
?
isopentanoyl-CoA + phosphate
95% of butanoylphosphate formation
486470
Clostridium acetobutylicum
isopentanoylphosphate + CoA
-
-
-
?
n-pentanoyl-CoA + phosphate
78% of butanoylphosphate formation
486470
Clostridium acetobutylicum
n-pentanoylphosphate + CoA
-
-
-
?
n-propionyl-CoA + phosphate
23% of butanoylphosphate formation
486470
Clostridium acetobutylicum
n-propionylphosphate + CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 31000, SDS-PAGE
Clostridium acetobutylicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
butanoylphosphate formation
Clostridium acetobutylicum
8.8
-
butanoyl-CoA formation
Clostridium acetobutylicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
9.5
butanoylphosphate formation, less than 50% of maximal activity above and below
Clostridium acetobutylicum
6.8
9.5
butanoyl-CoA formation, less than 50% of maximal activity above and below
Clostridium acetobutylicum
Other publictions for EC 2.3.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718679
Lehmann
New insights into the butyric ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
96
1325-1339
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
702786
Sillers
Aldehyde-alcohol dehydrogenase ...
Clostridium acetobutylicum
Biotechnol. Bioeng.
102
38-49
2009
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658942
Vazquez
Evidence of an association bet ...
Bacillus megaterium
Int. Microbiol.
6
127-129
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486476
Vazquez
Phosphotransbutyrylase express ...
Bacillus megaterium
Curr. Microbiol.
42
345-349
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486475
Liu
Exploitation of butyrate kinas ...
Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
53
545-552
2000
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486474
Walter
Studies of recombinant Clostri ...
Clostridium acetobutylicum
Ann. N. Y. Acad. Sci.
721
69-72
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486477
Oultram
Cloning and sequence analysis ...
Clostridium acetobutylicum, Clostridium acetobutylicum NCIMB 8052
Gene
131
107-112
1993
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486469
Thompson
Purification and properties of ...
Clostridium beijerinckii, Clostridium beijerinckii VPI 13437
Appl. Environ. Microbiol.
56
607-613
1990
-
-
-
-
-
-
-
5
-
-
2
2
-
3
-
-
1
-
-
-
-
2
8
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
2
2
-
-
-
1
-
-
-
2
8
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
486470
Wiesenborn
Phosphotransbutyrylase from Cl ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
55
317-322
1989
-
-
-
-
-
-
5
4
-
-
2
-
-
2
-
-
1
-
-
-
1
-
6
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
4
-
-
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
486471
Cary
Cloning and expression of Clos ...
Clostridium acetobutylicum
J. Bacteriol.
170
4613-4618
1988
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286050
Yarlett
Butyrate formation from glucos ...
Dasytricha ruminantium
Biochem. J.
228
187-192
1985
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286051
Miller
Enzymology of butyrate formati ...
Butyrivibrio fibrisolvens
J. Bacteriol.
138
99-104
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486468
Valentine
Purification and role of phosp ...
Clostridium butyricum, Clostridium kluyveri, Clostridium saccharoacetobutylicum, Clostridium sporogenes
J. Biol. Chem.
235
1948-1952
1960
-
-
-
-
-
-
-
-
-
4
-
-
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-