BRENDA - Enzyme Database show
show all sequences of 2.3.1.187

Enzymic and genetic basis for bacterial growth on malonate

Dimroth, P.; Hilbi, H.; Mol. Microbiol. 25, 3-10 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene mdcA, encoded in the malonate decarboxylase operon mdc, genetic organization
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60800
-
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
61200
-
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonate + acetyl-[acyl-carrier protein]
Klebsiella pneumoniae
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
malonate + acetyl-[acyl-carrier protein]
Malonomonas rubra
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
gene mdcA, encoded in the malonate decarboxylase operon mdc
-
Malonomonas rubra
-
gene madA, encoded in the malonate decarboxylase operon mad
-
Purification (Commentary)
Commentary
Organism
isolated soluble native transferase component of the malonate decarboxylase
Malonomonas rubra
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + acetyl-[acyl-carrier protein]
-
718124
Klebsiella pneumoniae
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
malonate + acetyl-[acyl-carrier protein]
-
718124
Malonomonas rubra
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
?
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Cloned(Commentary) (protein specific)
Commentary
Organism
gene mdcA, encoded in the malonate decarboxylase operon mdc, genetic organization
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60800
-
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
61200
-
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonate + acetyl-[acyl-carrier protein]
Klebsiella pneumoniae
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
malonate + acetyl-[acyl-carrier protein]
Malonomonas rubra
-
acetate + malonyl-[acyl-carrier protein]
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
isolated soluble native transferase component of the malonate decarboxylase
Malonomonas rubra
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + acetyl-[acyl-carrier protein]
-
718124
Klebsiella pneumoniae
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
malonate + acetyl-[acyl-carrier protein]
-
718124
Malonomonas rubra
acetate + malonyl-[acyl-carrier protein]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
Klebsiella pneumoniae
?
x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
Malonomonas rubra
General Information
General Information
Commentary
Organism
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Klebsiella pneumoniae
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Malonomonas rubra
additional information
the acetyl-S-ACP:malonate ACP transferase is a component of the malonate decarboxylase complex
Klebsiella pneumoniae
additional information
the acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase is a component of the malonate decarboxylase complex
Malonomonas rubra
General Information (protein specific)
General Information
Commentary
Organism
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Klebsiella pneumoniae
metabolism
to achieve decarboxylation of malonate, the key reaction in the decomposition of this compound, the substrate must be activated by the malonate decarboxylase to a malonyl thio-enzyme ester involving an activation module that comprises the acetylation of a specific thiol group of an acyl carrier protein, ACP, and the transfer of the ACP moiety to malonate, yielding malonyl-S-ACP and acetate. The malonyl-S-ACP is subsequently decarboxylated with regeneration of the acetyl-ACP. Malonate decarboxylation pathway, overview
Malonomonas rubra
additional information
the acetyl-S-ACP:malonate ACP transferase is a component of the malonate decarboxylase complex
Klebsiella pneumoniae
additional information
the acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase is a component of the malonate decarboxylase complex
Malonomonas rubra
Other publictions for EC 2.3.1.187
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
685645
Chohnan
Functions of malonate decarbox ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
67
214-217
2003
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686493
Hoenke
Formation of catalytically act ...
Klebsiella pneumoniae
Eur. J. Biochem.
259
181-187
1999
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686494
Koo
Functional evaluation of the g ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus MdcH
Eur. J. Biochem.
266
683-690
1999
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718124
Dimroth
Enzymic and genetic basis for ...
Klebsiella pneumoniae, Malonomonas rubra
Mol. Microbiol.
25
3-10
1997
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