BRENDA - Enzyme Database show
show all sequences of 2.3.1.187

Formation of catalytically active acetyl-S-malonate decarboxylase requires malonyl-coenzyme A:acyl carrier protein transacylase as auxiliary enzyme [corrected]

Hoenke, S.; Dimroth, P.; Eur. J. Biochem. 259, 181-187 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
preincubation with malonyl-CoA or methylmalonyl-CoA protects
Klebsiella pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
malonyl-CoA
pH 7.0
Klebsiella pneumoniae
0.016
-
methylmalonyl-CoA
pH 7.0
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + dephospho-CoA
-
686493
Klebsiella pneumoniae
CoA + malonyl-dephospho-CoA
-
-
-
?
methylmalonyl-CoA + dephospho-CoA
transfer proceeds via a covalent methylmalonyl-enzyme intermediate
686493
Klebsiella pneumoniae
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
additional information
no substrate: acetyl-CoA
686493
Klebsiella pneumoniae
?
-
-
-
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
preincubation with malonyl-CoA or methylmalonyl-CoA protects
Klebsiella pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
malonyl-CoA
pH 7.0
Klebsiella pneumoniae
0.016
-
methylmalonyl-CoA
pH 7.0
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + dephospho-CoA
-
686493
Klebsiella pneumoniae
CoA + malonyl-dephospho-CoA
-
-
-
?
methylmalonyl-CoA + dephospho-CoA
transfer proceeds via a covalent methylmalonyl-enzyme intermediate
686493
Klebsiella pneumoniae
CoA + methylmalonyl-dephospho-CoA
-
-
-
?
additional information
no substrate: acetyl-CoA
686493
Klebsiella pneumoniae
?
-
-
-
-
Other publictions for EC 2.3.1.187
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
685645
Chohnan
Functions of malonate decarbox ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
67
214-217
2003
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1
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2
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1
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-
686493
Hoenke
Formation of catalytically act ...
Klebsiella pneumoniae
Eur. J. Biochem.
259
181-187
1999
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686494
Koo
Functional evaluation of the g ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus MdcH
Eur. J. Biochem.
266
683-690
1999
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718124
Dimroth
Enzymic and genetic basis for ...
Klebsiella pneumoniae, Malonomonas rubra
Mol. Microbiol.
25
3-10
1997
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1
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2
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2
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4
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