Literature summary for 2.3.1.184 extracted from

Kambam, P.K.; Eriksen, D.T.; Lajoie, J.; Sayut, D.J.; Sun, L.
Altering the substrate specificity of RhlI by directed evolution (2009), ChemBioChem, 10, 553-558.

Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
E7K/F147L/P159E/E182G	strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration	Pseudomonas aeruginosa
E7K/F147L/V201M	strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine	Pseudomonas aeruginosa	-	N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine	-	ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine	Pseudomonas aeruginosa	production is only 1/15 of N-butanoyl homoserine lactone	N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine	-	ir

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine	-	Pseudomonas aeruginosa	N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine	-	ir
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine	production is only 1/15 of N-butanoyl homoserine lactone	Pseudomonas aeruginosa	N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine	-	ir

Synonyms

Synonyms	Comment	Organism
RhlI	-	Pseudomonas aeruginosa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)