Cloned (Comment) | Organism |
---|---|
gene rhlI, expression in Escherichia coli strain XL 1-Blue | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthioadenosine | 67% inhibition at 0.05 mM, 91% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
butyryl-S-adenosyl-L-methionine | 24% inhibition at 0.05 mM, 65% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
additional information | no inhibition by N-butyryl-L-homoserine-1,4-lactone, CoA, NADH, L-methionine, L-homocysteine, L-homoserine-1,4-lactone, pantothenate, L-homoserine, butyric acid, cerulenin, ATP, ADP, 4-hydroxybutyrate, butyryl-CoA, and apo-ACP | Pseudomonas aeruginosa | |
S-Adenosyl-D-homocysteine | 43% inhibition at 0.05 mM, 88% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
S-adenosyl-L-cysteine | 77% inhibition at 0.05 mM, 97% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
S-adenosyl-L-homocysteine | 62% inhibition at 0.05 mM, 91% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
sinefungin | 58% inhibition at 0.1 mM | Pseudomonas aeruginosa | |
[Acyl-carrier-protein] | holo-[acyl-carrier-protein], 2% inhibition at 0.05 mM, 55% inhibition at 0.5 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics of recombinant RhlI | Pseudomonas aeruginosa | |
0.006 | - |
butyryl-[acyl-carrier protein] | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa | |
0.008 | - |
hexanoyl-[acyl-carrier protein] | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa | |
0.014 | - |
S-adenosyl-L-methionine | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa | |
0.038 | - |
butyryl-S-adenosyl-L-methionine | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa | |
0.043 | - |
octanoyl-[acyl-carrier protein] | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa | |
0.23 | - |
butyryl-CoA | pH 7.8, 37°C, recombinant RhlI | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview | ? | - |
? | |
additional information | Pseudomonas aeruginosa PAO-JP1 | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene rhlI | - |
Pseudomonas aeruginosa PAO-JP1 | - |
gene rhlI | - |
Purification (Comment) | Organism |
---|---|
recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | RhlI shows a sequential, ordered ping-pong reaction mechanism with S-adenosyl-L-methionine binding first | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0021 | - |
purified recombinant RhlI | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview | Pseudomonas aeruginosa PAO-JP1 | ? | - |
? | |
additional information | substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates | Pseudomonas aeruginosa PAO-JP1 | ? | - |
? | |
S-adenosyl-L-methionine + butyryl-CoA | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA | - |
? | |
S-adenosyl-L-methionine + butyryl-S-adenosyl-L-methionine | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + S-adenosyl-L-methionine | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa PAO-JP1 | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa PAO-JP1 | 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa PAO-JP1 | 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl homoserine lactone synthase | - |
Pseudomonas aeruginosa |
More | the enzyme belongs to the LuxI family | Pseudomonas aeruginosa |
RhlI | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Pseudomonas aeruginosa |