Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C126S | site-directed mutagenesis, the mutant shows 81.5% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
C67S | site-directed mutagenesis, the mutant shows 1.9% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
C67S/C69S | site-directed mutagenesis, the mutant shows 0.8% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
C69S | site-directed mutagenesis, the mutant shows 30.4% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
C89S | site-directed mutagenesis, the mutant shows 63% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
D48N | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
D51N | site-directed mutagenesis, nearly inactive mutant | Pseudomonas aeruginosa |
E101K | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
E144K | site-directed mutagenesis, the mutant shows 43.5% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
E46K | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
F28L | site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
G159E | site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
G68D | site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
G68E | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
additional information | screening of diverse mutants constructrd by random mutagenesis, overview | Pseudomonas aeruginosa |
R104C | site-directed mutagenesis, nearly inactive mutant | Pseudomonas aeruginosa |
R104H | site-directed mutagenesis, nearly inactive mutant | Pseudomonas aeruginosa |
R24W | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
R71C | site-directed mutagenesis, the mutant shows 0.05% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
R71H | site-directed mutagenesis, inactive mutant | Pseudomonas aeruginosa |
S103E | site-directed mutagenesis, the mutant shows 5.4% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
W34G | site-directed mutagenesis, the mutant shows 0.10% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
W34Y | site-directed mutagenesis, the mutant shows 60% activity compared to the wild-type enzyme | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator | ? | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | Pseudomonas aeruginosa | - |
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P54291 | RhlI; gene rhlI | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | RhlI contains no active site cysteine, residues R24, F28, W34, E46, D48, D51, G68, C67, R71, E101, S103, and R104 are critical for catalytic activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator | Pseudomonas aeruginosa | ? | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | - |
Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acylhomoserine lactone synthase | - |
Pseudomonas aeruginosa |
autoinducer synthesis protein rhlI | - |
Pseudomonas aeruginosa |
RhlI | - |
Pseudomonas aeruginosa |