Cloned (Comment) | Organism |
---|---|
genes esaI and lasI, expression of wild-type and mutant enzymes in Escherichia coli | Pantoea stewartii |
Protein Variants | Comment | Organism |
---|---|---|
T140A | site-directed mutagenesis of EsaI, the mutant shows altered substrate acyl-chain length specificity compared to the wild-type enzyme | Pantoea stewartii |
T142A | site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme | Pantoea stewartii |
T142G | site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme | Pantoea stewartii |
T142S | site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme | Pantoea stewartii |
T144V | site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme | Pantoea stewartii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pantoea stewartii | EsaI and LasI produce N-acyl-L-homoserine lactones signaling molecules as autoinducers of quorum-sensing signaling and regulation of gene expression | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea stewartii | - |
genes esaI and lasI | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | Thr140 in the acyl-chain binding pocket directs the enzyme towards production of 3-oxohomoserine lactones and is involved in determination of substrate specificity of EsaI | Pantoea stewartii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | EsaI and LasI produce N-acyl-L-homoserine lactones signaling molecules as autoinducers of quorum-sensing signaling and regulation of gene expression | Pantoea stewartii | ? | - |
? | |
additional information | substrate specificities of wild-type and mutant EsaI and LasI, overview | Pantoea stewartii | ? | - |
? | |
S-adenosyl-L-methionine + 3-oxodecanoyl-[acyl-carrier protein] | substrate of wild-type and mutant LasI, overview | Pantoea stewartii | 5'-methylthioadenosine + N-3-oxodecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] | substrate of wild-type and mutant LasI, overview | Pantoea stewartii | 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] | substrate of wild-type EsaI and EsaI mutant T140A | Pantoea stewartii | 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxotetradecanoyl-[acyl-carrier protein] | substrate of wild-type and mutant LasI, overview | Pantoea stewartii | 5'-methylthioadenosine + N-3-oxotetradecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + 3-oxoundecanoyl-[acyl-carrier protein] | substrate of wild-type and mutant LasI, overview | Pantoea stewartii | 5'-methylthioadenosine + N-3-oxoundecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? | |
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] | preferred substrate of EsaI mutant T140A, no substrate of wild-type EsaI | Pantoea stewartii | 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL synthase | - |
Pantoea stewartii |
EsaI | - |
Pantoea stewartii |
LasI | - |
Pantoea stewartii |