Cloned (Comment) | Organism |
---|---|
gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0059 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.0074 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.2 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.283 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | Pseudomonas aeruginosa | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | structures of substrate, product, and transition state, overview | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + butyryl-CoA | substrate of RhlI, very low activity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1] | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2] | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3 | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3] | - |
? | |
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acylhomoserine lactone synthase | - |
Pseudomonas aeruginosa |
RhlI | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.05 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.35 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
0.46 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-profile | Pseudomonas aeruginosa |