Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli straoin BL21(DE3) | Pantoea stewartii subsp. stewartii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, vapor diffusion, 6 mg/ml protein in 0.1 M MES, pH 6.1, 14% PEG 4000, 6% 2-propanol, 0.03% 2-mercaptoethanol, 10 mM EDTA, and 0.5% NaN3, addition of agarose reduces the formation of a protein skin on the drop surface, X-ray diffraction structure determination and analysis at 1.14-1.19 A resolution, MAD phasing, modeling | Pantoea stewartii subsp. stewartii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea stewartii subsp. stewartii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Pantoea stewartii subsp. stewartii |
Synonyms | Comment | Organism |
---|---|---|
acyl-homoserinelactone synthase | - |
Pantoea stewartii subsp. stewartii |
AHL synthase | - |
Pantoea stewartii subsp. stewartii |
EsaI | - |
Pantoea stewartii subsp. stewartii |