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Literature summary for 2.3.1.183 extracted from

  • VanDrisse, C.M.; Hentchel, K.L.; Escalante-Semerena, J.C.
    Phosphinothricin acetyltransferases identified using in vivo, in vitro, and bioinformatic analyses (2016), Appl. Environ. Microbiol., 82, 7041-7051 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.183

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli C41(lambdaDE3) Geobacillus kaustophilus
expression in Escherichia coli C41(lambdaDE3) Deinococcus radiodurans

Protein Variants

Protein Variants Comment Organism
M55stop the active site of the mutant enzyme remains functional but is sufficiently altered to prevent acetylation of phosphinothricin Geobacillus kaustophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
0.005
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
0.017
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
0.089
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
0.121
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
0.198
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
0.316
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
0.721
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans Q9RV48
-
-
Deinococcus radiodurans ATCC 13939 Q9RV48
-
-
Geobacillus kaustophilus Q5KVT1
-
-
Geobacillus kaustophilus Q5L2F2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus kaustophilus
-
 Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-methionine sulfone
-
 Geobacillus kaustophilus CoA + N-acetyl-L-methionine sulfone
-
-
acetyl-CoA + L-methionine sulfone
-
 Deinococcus radiodurans CoA + N-acetyl-L-methionine sulfone
-
-
acetyl-CoA + L-methionine sulfone
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetyl-L-methionine sulfone
-
-
acetyl-CoA + L-methionine sulfoximine
-
 Geobacillus kaustophilus CoA + N-acetyl-L-methionine sulfoxime
-
-
acetyl-CoA + L-methionine sulfoximine
-
 Deinococcus radiodurans CoA + N-acetyl-L-methionine sulfoxime
-
-
acetyl-CoA + L-methionine sulfoximine
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetyl-L-methionine sulfoxime
-
-
acetyl-CoA + phosphinothricin
-
 Geobacillus kaustophilus CoA + N-acetylphosphinothricin
-
 ?
acetyl-CoA + phosphinothricin
-
 Deinococcus radiodurans CoA + N-acetylphosphinothricin
-
 ?
acetyl-CoA + phosphinothricin the active site of the mutant enzyme remains functional but is sufficiently altered to prevent acetylation of phosphinothricin Geobacillus kaustophilus CoA + N-acetylphosphinothricin
-
 ?
acetyl-CoA + phosphinothricin
-
 Deinococcus radiodurans ATCC 13939 CoA + N-acetylphosphinothricin
-
 ?

Synonyms

Synonyms Comment Organism
DR_1182
-
 Deinococcus radiodurans
GK0593
-
 Geobacillus kaustophilus
GK2920
-
 Geobacillus kaustophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
1
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
1
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
5
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
9
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
10
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
11
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
11
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
30
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
58.8
-
 L-methionine sulfoximine pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
60
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Deinococcus radiodurans
74.3
-
 L-methionine sulfoximine pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
112.4
-
 L-methionine sulfone pH 7.2, 30°C, mutant enzyme M55stop Geobacillus kaustophilus
200
-
 Phosphinothricin pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus
250
-
 L-methionine sulfone pH 7.2, 30°C, wild-type enzyme Geobacillus kaustophilus