Literature summary for 2.3.1.183 extracted from

Wang, C.; Lee, T.C.; Crowley, K.S.; Bell, E.
Purification of phosphinothricin acetyltransferase using Reactive Brown 10 affinity in a single chromatography step (2013), Protein Expr. Purif., 90, 129-134.

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Organism

Organism	UniProt	Comment	Textmining
Streptomyces hygroscopicus	P16426	-	-

Purification (Commentary)

Purification (Comment)	Organism
simple, time-saving, inexpensive and reproducible purification method employs a single chromatography step using a reactive dye resin, Reactive brown 10-agarose. Reactive brown 10 preferentially binds the phosphinothricin acetyltransferase protein, which can then be specifically released by acetyl-CoA. Phosphinothricin acetyltransferase protein can be purified to homogeneity from cottonseed with high recovery efficiency and enzymatically active	Streptomyces hygroscopicus

Purification (Comment)

Organism

simple, time-saving, inexpensive and reproducible purification method employs a single chromatography step using a reactive dye resin, Reactive brown 10-agarose. Reactive brown 10 preferentially binds the phosphinothricin acetyltransferase protein, which can then be specifically released by acetyl-CoA. Phosphinothricin acetyltransferase protein can be purified to homogeneity from cottonseed with high recovery efficiency and enzymatically active

Streptomyces hygroscopicus

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Release 2023.1 (January 2023)