Data extracted from this reference:
Organism
Substrates and Products (Substrate)
additional information
Lip3 is unable to modify either lipoyl domains or glycine cleavage system 3 when provided with free octanoate, ATP and MgCl2
737308
Saccharomyces cerevisiae
?
octanoyl-CoA + [glycine cleavage system 3]-L-lysine
737308
Saccharomyces cerevisiae
[glycine cleavage system 3]-N6-octanoyl-L-lysine + CoA
?
octanoyl-CoA + [oxoglutarate dehydrogenase]-L-lysine
737308
Saccharomyces cerevisiae
[oxoglutarate dehydrogenase]-N6-octanoyl-L-lysine + CoA
?
octanoyl-CoA + [pyruvate dehydrogenase]-L-lysine
737308
Saccharomyces cerevisiae
[pyruvate dehydrogenase]-N6-octanoyl-L-lysine + CoA
?
octanoyl-[acyl-carrier protein] + [glycine cleavage system 3]-L-lysine
737308
Saccharomyces cerevisiae
[glycine cleavage system 3]-N6-octanoyl-L-lysine + acyl-carrier protein
in vitro reaction
?
Substrates and Products (Substrate) (protein specific)
additional information
Lip3 is unable to modify either lipoyl domains or glycine cleavage system 3 when provided with free octanoate, ATP and MgCl2
737308
Saccharomyces cerevisiae
?
octanoyl-CoA + [glycine cleavage system 3]-L-lysine
737308
Saccharomyces cerevisiae
[glycine cleavage system 3]-N6-octanoyl-L-lysine + CoA
?
octanoyl-CoA + [oxoglutarate dehydrogenase]-L-lysine
737308
Saccharomyces cerevisiae
[oxoglutarate dehydrogenase]-N6-octanoyl-L-lysine + CoA
?
octanoyl-CoA + [pyruvate dehydrogenase]-L-lysine
737308
Saccharomyces cerevisiae
[pyruvate dehydrogenase]-N6-octanoyl-L-lysine + CoA
?
octanoyl-[acyl-carrier protein] + [glycine cleavage system 3]-L-lysine
737308
Saccharomyces cerevisiae
[glycine cleavage system 3]-N6-octanoyl-L-lysine + acyl-carrier protein
in vitro reaction
?
General Information
physiological function
octanoyltransferase Lip2 specifically modifies glycine cleavage system 3 using octanoyl-ACP from mitochondrial FA biosynthesis. When all glycine cleavage system 3 is octanoylated, octanoyl-ACP accumulates. An octanoyl-ACP: CoA transferase transfers the octanoyl moiety to CoA, providing substrate for octanoyltransferase Lip3 to modify pyruvate dehydrogenase and oxoglutarate dehydrogenase. Complementation of the Escherichia coli DELTAlipB DELTAlplA strain by expression of Lip3 requires a host-encoded acyl-CoA synthase
Saccharomyces cerevisiae
General Information (protein specific)
physiological function
octanoyltransferase Lip2 specifically modifies glycine cleavage system 3 using octanoyl-ACP from mitochondrial FA biosynthesis. When all glycine cleavage system 3 is octanoylated, octanoyl-ACP accumulates. An octanoyl-ACP: CoA transferase transfers the octanoyl moiety to CoA, providing substrate for octanoyltransferase Lip3 to modify pyruvate dehydrogenase and oxoglutarate dehydrogenase. Complementation of the Escherichia coli DELTAlipB DELTAlplA strain by expression of Lip3 requires a host-encoded acyl-CoA synthase
Saccharomyces cerevisiae
Other publictions for EC 2.3.1.181
726128
Ewald
Two redundant octanoyltransfer ...
Arabidopsis thaliana
Plant Biol.
16
35-42
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
737026
Ewald
Lipoate-protein ligase and oct ...
Arabidopsis thaliana
Plant Physiol.
165
978-990
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737308
Hermes
The role of the Saccharomyces ...
Saccharomyces cerevisiae
Yeast
30
415-427
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712504
Hassan
Protein-protein interactions i ...
Escherichia coli
J. Biol. Chem.
286
8263-8276
2011
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718133
Martin
A novel two-gene requirement f ...
Bacillus subtilis
Mol. Microbiol.
80
335-349
2011
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
718134
Christensen
A novel amidotransferase requi ...
Bacillus subtilis
Mol. Microbiol.
80
350-363
2011
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
711217
Christensen
Lipoic acid synthesis: a new f ...
Bacillus subtilis
Biochemistry
49
10024-10036
2010
-
-
1
-
4
-
-
-
-
-
1
1
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
704311
Hermes
Scavenging of cytosolic octano ...
Escherichia coli
J. Bacteriol.
191
6796-6803
2009
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706425
Guenther
Knockout studies reveal an imp ...
Plasmodium falciparum
PLoS ONE
4
e5510
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
688368
Fujiwara
Crystal structure of bovine li ...
Bos taurus
J. Mol. Biol.
371
222-234
2007
-
-
1
1
-
-
-
-
1
-
-
1
-
4
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
676872
Ma
The Mycobacterium tuberculosis ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
103
8662-8667
2006
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661184
Zhao
The reaction of LipB, the octa ...
Escherichia coli
Biochemistry
44
16737-16746
2005
-
-
1
-
3
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663308
Nesbitt
Expression, purification, and ...
Escherichia coli
Protein Expr. Purif.
39
269-282
2005
2
-
1
-
-
-
1
2
-
-
2
1
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
2
1
1
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
-
1
-
2
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
662002
Jordan
The Escherichia coli lipB gene ...
Escherichia coli
J. Bacteriol.
185
1582-1589
2003
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663062
Wada
Lipoic acid metabolism in Arab ...
Arabidopsis thaliana
Plant Cell Physiol.
42
650-656
2001
-
-
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662098
Jordan
A new metabolic link. The acyl ...
Escherichia coli, Neurospora crassa, Pisum sativum
J. Biol. Chem.
272
17903-17906
1997
-
-
-
-
-
-
-
-
2
-
-
3
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661982
vanden Boom
Lipoic acid metabolism in Esch ...
Escherichia coli
J. Bacteriol.
173
6411-6420
1991
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-