Cloned (Comment) | Organism |
---|---|
gene fabH, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Synechococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the Synechococcus FabH is used for replacement of its counterpart in the reconstituted Escherichia coli fatty acid synthase system, the resulting complex is strongly limited in FabH activity. The introduction of the Escherichia coli FabH enzyme into the Synechococcus fatty acid synthase system virtually eliminates its dependence on the FabH subunit | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | Synechococcus sp. | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | B1XPH5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography, followed by ultrafiltration | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Synechococcus sp. | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | Product profile analysis of FabH from Synechococcus sp. strain PCC 7002, overview. t´The major products are lauric acid, myristic acid, palmitic acid, and stearic acid | Synechococcus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FabH | - |
Synechococcus sp. |
initiation ketosynthase | - |
Synechococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 25 | assay at room temperature | Synechococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Synechococcus sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the initiation ketosynthase FabH is the sole rate-limiting enzyme of the fatty acid synthesis of Synechococcus sp. PCC 700. Reconstitution of the fatty acid synthase system, FAS. FabH ketosynthase initiates product synthesis by condensing malonyl-ACP with acetyl-CoA to form acetoacetyl-ACP | Synechococcus sp. |