Cloned (Comment) | Organism |
---|---|
expression of selenomethionine-labeled enzyme in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme, 0.002 ml of 13 mg/ml protein in 20 mM Tris, pH 7.5, 50 mM NaCl, 10 mM DTT, and 4 mM acetyl-CoA, versus 0.002 ml reservoir solution containing 12-14% PEG 4000, 40 mM Bis-Tris propane, pH 7.0, and 80 mM magnesium acetate, overnight, X-ray diffraction structure determination and analysis at 1.9 A resolution | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33516 | - |
2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
33889 | - |
2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
65000 | - |
selenomethionine-labeled recombinant enzyme, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Escherichia coli | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene fabH | - |
Purification (Comment) | Organism |
---|---|
recombinant selenomethionine-labeled enzyme by ion exchange, hydroxylapatite, and affinity chromatography, and gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | the enzyme shows also acyl-CoA-[acyl-carrier-protein] transacylase activity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33516, amino acid sequence calculation, 2 * 33889, selenomethionine-labeled recombinant enzyme, mass spectrometry | Escherichia coli |
More | noncovalent homodimer by dynamic light scattering | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ACP synthase III | - |
Escherichia coli |
beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
FabH | - |
Escherichia coli |
KAS III | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
unfolding of the recombinant selenomethionine-labeld enzyme starts above | Escherichia coli |
70 | - |
about, Tm, recombinant selenomethionine-labeld enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing, recombinant enzyme | - |
5.1 |