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Literature summary for 2.3.1.18 extracted from

  • Lewendon, A.; Ellis, J.; Shaw, W.V.
    Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product (1995), J. Biol. Chem., 270, 26326-26331.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
H115A kcat highly decreased, histidyl residue has an important catalytic role Escherichia coli
S162A kcat increased, Ser162 not catalytically important Escherichia coli
W139F kcat 10fold decreased Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide complete inactivation Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
acetyl-CoA phenyl-beta-D-galactopyranoside Escherichia coli
0.065
-
acetyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
0.082
-
acetyl-CoA o-nitrophenyl-beta-D-galactopyranoside Escherichia coli
0.098
-
propionyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
0.104
-
acetyl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
0.221
-
butyryl-CoA p-nitrophenyl-beta-D-galactopyranoside Escherichia coli
0.266
-
acetyl-CoA p-nitrophenyl-beta-D-lactopyranoside Escherichia coli
25.2
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: butyrylCoA Escherichia coli
40.2
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
45.6
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: propionyl-CoA Escherichia coli
57.9
-
p-nitrophenyl-alpha-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
60.2
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
63.4
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
63.4
-
phenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
67.9
-
o-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + acetyl-CoA
-
Escherichia coli 4-nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside + CoA
-
?
acetyl-CoA + beta-D-phenylgalactoside
-
Escherichia coli CoA + 6-O-acetyl-beta-D-phenylgalactoside
-
?
acetyl-CoA + o-nitrophenyl-beta-D-galactopyranoside
-
Escherichia coli CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucopyranoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-glucopyranoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-lactopyranoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactopyranoside
-
?
butyryl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-butyryl-beta-D-galactopyranoside
-
?
additional information enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside Escherichia coli ?
-
?
propionyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-propionyl-beta-D-galactopyranoside
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.054
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: butyryl-CoA Escherichia coli
1.42
-
4-nitrophenyl alpha-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
1.84
-
p-nitrophenyl-beta-D-glucopyranoside acetyl donor: acetyl-CoA Escherichia coli
1.84
-
p-nitrophenyl-beta-D-lactopyranoside acetyl donor: acetyl-CoA Escherichia coli
5.8
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: propionyl-CoA Escherichia coli
8.9
-
o-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
13
-
phenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli
14.7
-
p-nitrophenyl-beta-D-galactopyranoside acetyl donor: acetyl-CoA Escherichia coli